Lamin B2 Rekombinanter Antikörper

• Featured Product
• KD/KO Validated

Lamin B2 Rekombinant Antikörper für WB, IHC, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

230184E7

Kat-Nr. : 82801-2-RR

Synonyme

LMNB2, 230184E7, LAMB2, Lamin-B2, LMN2

---

• 

  WB analysis using 82801-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82801-2-RR (Lamin B2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 82801-2-RR

  WB result of Lamin B2 antibody (82801-2-RR; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Lamin B2 transfected A549 cells.

• 

  IHC staining of human stomach cancer using 82801-2-RR

  Immunohistochemical analysis of paraffin-embedded stomach cancer slide using 82801-2-RR (Lamin B2 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 82801-2-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Lamin B2 antibody (82801-2-RR, Clone: 230184E7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HepG2 using 82801-2-RR

  (blue)1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Anti-Human Lamin B2 (82801-2-RR, Clone:230184E7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:166(red), or 0.25 ug rabbit IgG isotype control. Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

• 

  Affinity and Kinetic Characterization of 82801-2-RR

  Biolayer interferometry (BLl) kinetic assays of 82801-2-RR against Human Lamin B2 were performed. The affinity constant is 2.81 nM.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A549-Zellen, COLO 320-Zellen, HL-60-Zellen, HT-1080.Zellen, Jurkat-Zellen
• Erfolgreiche Detektion in IHC: humanes Magenkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen
• Erfolgreiche Detektion in FC (Intra): HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunhistochemie (IHC): IHC : 1:400-1:1600
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

82801-2-RR bindet in WB, IHC, IF/ICC, FC (Intra), ELISA Lamin B2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Lamin B2 fusion protein Ag1335
• Vollständiger Name: lamin B2
• Berechnetes Molekulargewicht: 68 kDa
• Beobachtetes Molekulargewicht: 68 kDa
• GenBank-Zugangsnummer: BC006551
• Gene symbol: Lamin B2
• Gene ID (NCBI): 84823
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Lamins are nuclear membrane structural components that are important in structural integrity of the nucleus and may also interact with chromatin (PMID: 33033404). Research studies show that lamin B2 knockout mice exhibit neuronal developmental defects and that both proteins are essential for typical brain development (PMID: 20145110). Mutations in Lamin B2 can result in a susceptibility to developing acquired partial lipodystrophy, a rare disorder characterized by the progressive loss of subcutaneous fat in a bilaterally symmetrical fashion (PMID: 16826530).