LCAT Rekombinanter Antikörper

LCAT Rekombinant Antikörper für WB, Cytometric bead array, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus, Ratte

Anwendung

WB, Cytometric bead array, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

250320E2

Kat-Nr. : 86041-1-PBS

Synonyme

1-alkyl-2-acetylglycerophosphocholine esterase, EC:2.3.1.43, EC:3.1.1.47, Lecithin-cholesterol acyltransferase, PAF acetylhydrolase

---

• 

  WB analysis using 86041-1-RR (same clone as 86041-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86041-1-RR (LCAT antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86041-1-PBS in a different storage buffer formulation.

• 

  Sample test of MP02258-1

  The mean LCAT concentration was determined to be 863.3 ng/mL in mouse serum.

• 

  Cytometric bead array standard curve of MP02258-1

  Cytometric bead array standard curve of MP02258-1, MOUSE LCAT Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86041-2-PBS. Detection antibody: 86041-1-PBS. Standard: Eg2756. Range: 0.781-100 ng/mL.

---

Geprüfte Anwendungen

Produktinformation

86041-1-PBS bindet in WB, Cytometric bead array, Indirect ELISA, Sample test LCAT und zeigt Reaktivität mit Maus, Ratten

• Getestete Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: LCAT fusion protein Eg2756
• Vollständiger Name: lecithin cholesterol acyltransferase
• Berechnetes Molekulargewicht: 50 kDa
• Beobachtetes Molekulargewicht: 55-65 kDa
• GenBank-Zugangsnummer: NM_008490.2
• Gene symbol: Lcat
• Gene ID (NCBI): 16816
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LCAT (Lecithin-cholesterol acyltransferase) is a lipoprotein-associated enzyme responsible for the synthesis of most of the plasma cholesteryl esters (CE), and as a consequence, for the maturation of high density-lipoproteins (HDL). LCAT is a 416 amino acid protein that binds to lipoproteins or is present in lipid-free form in plasma and is secreted by the liver in humans (PMID: 34256778, 26607351). Both mice and rabbits transgenic for human LCAT have marked increases in plasma HDL cholesterol and apolipoprotein (apo) A-I levels (PMID: 9054454).