Leptin Rekombinanter Antikörper

Leptin Rekombinant Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

240368E6

Kat-Nr. : 83655-3-RR

Synonyme

Lep, OB, 240368E6

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  WB analysis of rat adipose using 83655-3-RR

  Rat adipose tissue was subjected to SDS PAGE followed by western blot with 83655-3-RR (Leptin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 83655-3-RR

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83655-3-RR (Leptin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 83655-3-RR

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83655-3-RR (Leptin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 83655-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83655-3-RR against Rat Leptin were performed. The affinity constant is 3.54 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: rat adipose tissue
• Erfolgreiche Detektion in IHC: humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83655-3-RR bindet in WB, IHC, ELISA Leptin und zeigt Reaktivität mit Ratten

• Getestete Reaktivität: Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Fusionsprotein
• Vollständiger Name: leptin
• Berechnetes Molekulargewicht: 19 kDa
• Beobachtetes Molekulargewicht: 15-16 kDa
• GenBank-Zugangsnummer: NM_013076.3
• Gene symbol: Leptin
• Gene ID (NCBI): 25608
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Leptin is a protein that is secreted by white adipocytes, and which plays a major role in the regulation of body weight. Leptin is the most critical hormone in the homeostatic regulation of energy balance among those so far discovered. Leptin primarily acts on the neurons of the mediobasal part of hypothalamus to regulate food intake, thermogenesis, and the blood glucose level. Leptin also has several endocrine functions, and is involved in the regulation of immune and inflammatory responses, hematopoiesis, angiogenesis and wound healing. Mutations in this gene and/or its regulatory regions cause severe obesity, and morbid obesity with hypogonadism. Leptin has also been linked to type 2 diabetes mellitus development.