Lin28A-specific Polyklonaler Antikörper

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• KD/KO Validated

Lin28A-specific Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(13)

Rezensionen (2)

Kat-Nr. : 16177-1-AP

Synonyme

LIN28A, Lin-28A, LIN28, Lin 28A, LIN 28

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  WB analysis of hESC using 16177-1-AP

  hESC cells were subjected to SDS PAGE followed by western blot with 16177-1-AP (Lin28A-specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NCCIT using 16177-1-AP

  NCCIT cell were subjected to SDS PAGE followed by western blot with 16177-1-AP (Lin28A-specific Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 16177-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16177-1-AP (Lin28A-specific antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 16177-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 16177-1-AP (Lin28A-specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 16177-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 16177-1-AP (Lin28A-specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human small intestine using 16177-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine using 16177-1-AP (Lin28A-specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 16177-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16177-1-AP (Lin28A-specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 16177-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16177-1-AP (Lin28A-specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 16177-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16177-1-AP (Lin28A-specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 16177-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16177-1-AP (Lin28A-specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 16177-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16177-1-AP (Lin28A-specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 16177-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16177-1-AP (Lin28A-specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human spleen using 16177-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16177-1-AP (Lin28A-specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 16177-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16177-1-AP (Lin28A-specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung using 16177-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 16177-1-AP (Lin28A-specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung using 16177-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 16177-1-AP (Lin28A-specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human ovary using 16177-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16177-1-AP (Lin28A-specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human ovary using 16177-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16177-1-AP (Lin28A-specific antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: hESC-Zellen, humanes Hirngewebe, humanes Herzgewebe, Mauslebergewebe
• Erfolgreiche Detektion in IHC: humanes Dünndarmgewebe, humanes Hirngewebe, humanes Herzgewebe, humanes Lungengewebe, humanes Eierstockgewebe, humanes Milzgewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 11 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Produktinformation

16177-1-AP bindet in WB, IHC, IF, ELISA Lin28A-specific und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: lin-28 homolog (C. elegans)
• Berechnetes Molekulargewicht: 23 kDa
• Beobachtetes Molekulargewicht: 28 kDa
• GenBank-Zugangsnummer: NM_024674
• Gene symbol: LIN28
• Gene ID (NCBI): 79727
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LIN28 is one of the four key human factors (OCT4, SOX2, NANOG and LIN28) used to reprogram human fibroblasts to an embryonic stem (ES) cell-like state known as the induced pluripotent stem (Ips) cell. LIN28 is a marker of undifferentiated human embryonic stem cells and a cytoplasmic mRNA-binding protein that binds to and enhances the translation of the IGF2 mRNA. LIN28 has also been shown to bind to the let-7 pre-miRNA and block production of the mature let-7 microRNA in mouse embryonic stem cells. Affinity purified rabbit anti-LIN28 can be used to demonstrate pluripotency of ES and Ips cells, and to detect LIN28 transgene expression in the process of reprogramming. This antibody is a rabbit polyclonal antibody raised against a peptide mapping within human LIN28.This antibody recognize Lin28A specifically.

Publikationen

Species	Application	Title
human	WB	Cell Death Differ; LIN28A enhances regenerative capacity of human somatic tissue stem cells via metabolic and mitochondrial reprogramming.; Authors - Kelvin Pieknell
human	WB	Cancer Res; Activation of the Lin28/let-7 Axis by Loss of ESE3/EHF Promotes a Tumorigenic and Stem-like Phenotype in Prostate Cancer.; Authors - Domenico Albino
human	WB	EMBO Rep; Lin28 enhances de novo fatty acid synthesis to promote cancer progression via SREBP-1.; Authors - Yang Zhang ; KD Validated
human	WB	EMBO Rep; The human FOXM1 homolog promotes basal progenitor cell proliferation and cortical folding in mouse.; Authors - Wenwen Wang
human	WB	Cell Death Dis; Long noncoding RNA TUG1 facilitates osteogenic differentiation of periodontal ligament stem cells via interacting with Lin28A.; Authors - Qin He
Mouse	WB	Oncotarget; PMID: 29552298; Authors - Dawei Lei

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Carolina (Verified Customer) (09-26-2019)

Antibody was more specific than the previous Lin28A antibody.

• Applications: Immunohistochemistry,
• Primary Antibody Dilution: 1:20
• Cell Tissue Type: Human Testis

FH

KUNHUA (Verified Customer) (02-01-2019)

Got a very strong band at 25-37kd with 1:1000 concentration.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: human erythroid progenitor