Lipocalin-2/NGAL Rekombinanter Antikörper

• Featured Product
• KD/KO Validated

Lipocalin-2/NGAL Rekombinant Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2G1

Kat-Nr. : 82841-1-PBS

Synonyme

LCN2, lipocalin 2, NGAL, Lcn 2, HNL

---

• 

  WB analysis using 82841-1-RR (same clone as 82841-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82841-1-RR (Lipocalin-2/NGAL antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82841-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HT-29 using 82841-1-RR (same clone as 82841-1-PBS)

  WB result of NGAL antibody (82841-1-RR; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-NGAL transfected HT-29 cells. This data was developed using the same antibody clone with 82841-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human intrahepatic cholangiocarcinoma using 82841-1-RR (same clone as 82841-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 82841-1-RR (NGAL antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82841-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 82841-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82841-1-RR against Human NGAL were performed. The affinity constant is 0.776 nM.

---

Geprüfte Anwendungen

Produktinformation

82841-1-PBS bindet in WB, IHC, Indirect ELISA Lipocalin-2/NGAL und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: lipocalin 2
• Berechnetes Molekulargewicht: 23kd
• Beobachtetes Molekulargewicht: 22-25 kDa
• GenBank-Zugangsnummer: NP_005555.2
• Gene symbol: NGAL/LCN2
• Gene ID (NCBI): 3934
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Lipocalin-2 (LCN-2) is a adipocytokine also referred to as neutrophil gelatinase-associated lipocalin (NGAL). Lipocalin-2 is a circulatory protein responsible for the transportation of small and hydrophobic molecules to target organs. Lipocalin-2 is used as a biomarker for acute and chronic renal injury. It is present in a large variety of cells including neutrophil, hepatocytes, lung, bone marrow, adipose tissue, macrophages, thymus, non-neoplastic breast duct, prostate, and renal cells. Different functions have been associated with Lipocalin-2, including antibacterial, anti-inflammatory, and protection against cell and tissue stress (PMID:34463264).