Lipocalin-2/NGAL Rekombinanter Antikörper

Lipocalin-2/NGAL Rekombinant Antikörper für WB, IHC, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus, Ratte

Anwendung

WB, IHC, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

240792C12

Kat-Nr. : 83853-2-PBS

Synonyme

Lcn2, Lipocalin2/NGAL, 240792C12, Alpha-2-microglobulin-related protein, Alpha-2U globulin-related protein

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  WB analysis using 83853-2-RR (same clone as 83853-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83853-2-RR (Lipocalin-2/NGAL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83853-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse spleen using 83853-2-RR (same clone as 83853-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83853-2-RR (Lipocalin-2/NGAL antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83853-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse spleen using 83853-2-RR (same clone as 83853-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83853-2-RR (Lipocalin-2/NGAL antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83853-2-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83853-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83853-2-RR against Rat Lipocalin-2/Ngal were performed. The affinity constant is 0.195 nM.

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Geprüfte Anwendungen

Produktinformation

83853-2-PBS bindet in WB, IHC, Cytometric bead array, Indirect ELISA Lipocalin-2/NGAL und zeigt Reaktivität mit Maus, Ratten

• Getestete Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Lipocalin-2/NGAL fusion protein Eg1022
• Vollständiger Name: lipocalin 2
• Berechnetes Molekulargewicht: 22kDa
• Beobachtetes Molekulargewicht: 22-25 kDa
• Gene symbol: Lipocalin-2/NGAL
• Gene ID (NCBI): 170496
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Neutrophil gelatinase-associated lipocalin (NGAL, also known as lipocalin-2, siderocalin, or LCN2) is a small molecule of almost 25 kDa that belongs to the well-defined superfamily of proteins called lipocalins. NGAL was initially found in activated neutrophils, under its role as an innate antibacterial factor. However, it was subsequently shown that many other types of cells, including those in the kidney tubule, may produce NGAL in response to various injuries. NGAL may become one of the most promising next-generation biomarkers in clinical nephrology and beyond. This antibody recognizes rat Lipocalin-2/Ngal.