Lipocalin-2/NGAL Rekombinanter Antikörper

Lipocalin-2/NGAL Rekombinant Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

240792C12

Kat-Nr. : 83853-2-RR

Synonyme

Lcn2, NGAL, Lipocalin-2, lipocalin 2, 240792C12

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  WB analysis using 83853-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83853-2-RR (Lipocalin-2/NGAL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse spleen using 83853-2-RR

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83853-2-RR (Lipocalin-2/Ngal antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse spleen using 83853-2-RR

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83853-2-RR (Lipocalin-2/Ngal antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 83853-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83853-2-RR against Rat Lipocalin-2/Ngal were performed. The affinity constant is 0.195 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: 3T3-L1-Zellen, Maushodengewebe, Rattenlungengewebe
• Erfolgreiche Detektion in IHC: Mausmilzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83853-2-RR bindet in WB, IHC, ELISA Lipocalin-2/NGAL und zeigt Reaktivität mit Maus, Ratten

• Getestete Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: lipocalin 2
• Berechnetes Molekulargewicht: 22kDa
• Beobachtetes Molekulargewicht: 22-25 kDa
• Gene symbol: Lipocalin-2/NGAL
• Gene ID (NCBI): 170496
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Neutrophil gelatinase-associated lipocalin (NGAL, also known as lipocalin-2, siderocalin, or LCN2) is a small molecule of almost 25 kDa that belongs to the well-defined superfamily of proteins called lipocalins. NGAL was initially found in activated neutrophils, under its role as an innate antibacterial factor. However, it was subsequently shown that many other types of cells, including those in the kidney tubule, may produce NGAL in response to various injuries. NGAL may become one of the most promising next-generation biomarkers in clinical nephrology and beyond. This antibody recognizes rat Lipocalin-2/Ngal.