Livin Polyklonaler Antikörper

Livin Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 27543-1-PBS

Synonyme

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  WB analysis using 27543-1-AP (same clone as 27543-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 27543-1-AP (Livin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27543-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A431 using 27543-1-AP (same clone as 27543-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 27543-1-AP (Livin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27543-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A375 using 27543-1-AP (same clone as 27543-1-PBS)

  A375 cells were subjected to SDS PAGE followed by western blot with 27543-1-AP (Livin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27543-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 27543-1-AP (same clone as 27543-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27543-1-AP (Livin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27543-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 27543-1-AP (same clone as 27543-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27543-1-AP (Livin antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27543-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

27543-1-PBS bindet in WB, IHC, Indirect ELISA Livin und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Livin fusion protein Ag26737
• Vollständiger Name: baculoviral IAP repeat-containing 7
• Berechnetes Molekulargewicht: 33 kDa
• Beobachtetes Molekulargewicht: 33 kDa
• GenBank-Zugangsnummer: BC014475
• Gene symbol: Livin
• Gene ID (NCBI): 79444
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BIRC7 also known as Livin, is one of the family members of the inhibitor of apoptosis protein (IAP). BIRC7 contains a BIR domain that has been shown to bind and inhibit caspases-3, −7 and −9, playing a key role in inhibiting apoptosis. Overexpression of BIRC7 in tumors has been linked with increased chemo- and radio-resistance, recurrence, and decreased patient survival. BIRC7 is closely associated with melanoma disease progression, apoptosis resistance, and chemotherapeutic drug resistance.