Lumican Polyklonaler Antikörper

Lumican Polyklonal Antikörper für WB, IF-P, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 10677-1-PBS

Synonyme

LUM, SLRR2D, LDC, KSPG lumican

---

• 

  WB analysis of Jurkat using 10677-1-AP (same clone as 10677-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10677-1-AP (Lumican antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10677-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 10677-1-AP (same clone as 10677-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10677-1-AP (Lumican antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10677-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 10677-1-AP (same clone as 10677-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10677-1-AP (Lumican antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10677-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse spleen using 10677-1-AP (same clone as 10677-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using Lumican antibody (10677-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10677-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

10677-1-PBS bindet in WB, IF-P, Indirect ELISA Lumican und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Lumican fusion protein Ag1106
• Vollständiger Name: lumican
• Berechnetes Molekulargewicht: 38 kDa
• Beobachtetes Molekulargewicht: 50-90 kDa
• GenBank-Zugangsnummer: BC007038
• Gene symbol: Lumican
• Gene ID (NCBI): 4060
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Lumican (LUM), a member of the small leucine-rich proteoglycan (SLRP) family, is one of the major extracellular components in interstitial collagenous matrices of the corneal stroma, aorta, skin, skeletal muscle, lung, kidney, bone, cartilage, and intervertebral discs. SLRPs constitute an important fraction of noncollagenous extracellular matrix proteins and have important effects on cell behavior. Lumican regulates collagenous matrix assembly as a keratan sulfate proteoglycan in the cornea and may exist as a glycoprotein in the connective tissues of other organs. Posttranslational modifications lumican goes through can increase its apparent molecular weight to 50-90 kDa. Studies show that lumican participates in the maintenance of tissue homeostasis and modulates cellular functions including cell proliferation, migration, and differentiation. (PMID: 18949819; 22252641; 9761754; 22365843)