Lumican Rekombinanter Antikörper

Lumican Rekombinant Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242298G6

Kat-Nr. : 85629-1-PBS

Synonyme

Keratan sulfate proteoglycan lumican, KSPG lumican, Lcn, Ldc, Lum

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  WB analysis of Recombinant protein using 85629-1-RR (same clone as 85629-1-PBS)

  Recombinant protein were subjected to SDS PAGE followed by western blot with 85629-1-RR (Lumican antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85629-1-PBS in a different storage buffer formulation.

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  WB analysis using 85629-1-RR (same clone as 85629-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85629-1-RR (Lumican antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85629-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 85629-1-RR (same clone as 85629-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85629-1-RR (Lumican antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85629-1-PBS in a different storage buffer formulation.

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  IHC staining of rat colon using 85629-1-RR (same clone as 85629-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 85629-1-RR (Lumican antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85629-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat colon using 85629-1-RR (same clone as 85629-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 85629-1-RR (Lumican antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85629-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

85629-1-PBS bindet in WB, IHC, Indirect ELISA Lumican und zeigt Reaktivität mit Maus, Ratten

• Getestete Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Lumican fusion protein Eg3410
• Vollständiger Name: lumican
• Berechnetes Molekulargewicht: 38 kDa
• Beobachtetes Molekulargewicht: 50-90 kDa
• GenBank-Zugangsnummer: NM_031050.1
• Gene symbol: Lum
• Gene ID (NCBI): 81682
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

umican (LUM), a member of the small leucine-rich proteoglycan (SLRP) family, is one of the major extracellular components in interstitial collagenous matrices of the corneal stroma, aorta, skin, skeletal muscle, lung, kidney, bone, cartilage, and intervertebral discs. SLRPs constitute an important fraction of noncollagenous extracellular matrix proteins and have important effects on cell behavior. Lumican regulates collagenous matrix assembly as a keratan sulfate proteoglycan in the cornea and may exist as a glycoprotein in the connective tissues of other organs. Posttranslational modifications lumican goes through can increase its apparent molecular weight to 50-90 kDa. Studies show that lumican participates in the maintenance of tissue homeostasis and modulates cellular functions including cell proliferation, migration, and differentiation. (PMID: 18949819; 22252641; 9761754; 22365843)