Lumican Rekombinanter Antikörper

Lumican Rekombinant Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

242298G6

Kat-Nr. : 85629-1-RR

Synonyme

Keratan sulfate proteoglycan lumican, KSPG lumican, Lcn, Ldc, Lum

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  WB analysis of Recombinant protein using 85629-1-RR

  Recombinant protein were subjected to SDS PAGE followed by western blot with 85629-1-RR (Lumican antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 85629-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85629-1-RR (Lumican antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 85629-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85629-1-RR (Lumican antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of rat colon using 85629-1-RR

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 85629-1-RR (Lumican antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat colon using 85629-1-RR

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 85629-1-RR (Lumican antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Recombinant protein, Maushautgewebe, Rattenherzgewebe, Rattenlungengewebe, Rattenhautgewebe
• Erfolgreiche Detektion in IHC: Ratten-Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:2000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

85629-1-RR bindet in WB, IHC, ELISA Lumican und zeigt Reaktivität mit Maus, Ratten

• Getestete Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Lumican fusion protein Eg3410
• Vollständiger Name: lumican
• Berechnetes Molekulargewicht: 38 kDa
• Beobachtetes Molekulargewicht: 50-90 kDa
• GenBank-Zugangsnummer: NM_031050.1
• Gene symbol: Lum
• Gene ID (NCBI): 81682
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

umican (LUM), a member of the small leucine-rich proteoglycan (SLRP) family, is one of the major extracellular components in interstitial collagenous matrices of the corneal stroma, aorta, skin, skeletal muscle, lung, kidney, bone, cartilage, and intervertebral discs. SLRPs constitute an important fraction of noncollagenous extracellular matrix proteins and have important effects on cell behavior. Lumican regulates collagenous matrix assembly as a keratan sulfate proteoglycan in the cornea and may exist as a glycoprotein in the connective tissues of other organs. Posttranslational modifications lumican goes through can increase its apparent molecular weight to 50-90 kDa. Studies show that lumican participates in the maintenance of tissue homeostasis and modulates cellular functions including cell proliferation, migration, and differentiation. (PMID: 18949819; 22252641; 9761754; 22365843)