Ly-6C Rekombinanter Antikörper

Ly-6C Rekombinant Antikörper für FC

Wirt / Isotyp

Ratte / IgG2a

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

HK1.4

Kat-Nr. : 65669-1-TR

Synonyme

Ly6c, Ly 6C, Ly6c1

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  FC experiment of mouse bone marrow cells using 65669-1-TR

  1x10^6 mouse bone marrow cells were surface stained with 0.5 ug Anti-Mouse Ly-6C (HK1.4) Rat IgG2a RecAb (65669-1-TR, Clone: HK1.4) or Rat IgG2a Isotype Control Recombinant Antibody (98427-1-TR, Clone: 250862A1), and FITC anti-Rat IgG2a antibody. Cells were then stained with APC Anti-Mouse CD11b (M1/70) (APC-65055, Clone: M1/70). Cells were not fixed.

• 

  FC experiment of mouse bone marrow cells using 65669-1-TR

  1x10^6 mouse bone marrow cells were surface stained with 0.5 ug Anti-Mouse Ly-6C (HK1.4) Rat IgG2a RecAb (65669-1-TR, Clone: HK1.4) (red) or Rat IgG2a Isotype Control Recombinant Antibody (98427-1-TR, Clone: 250862A1) (blue), and FITC anti-Rat IgG2a antibody. Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Maus-Knochenmarkzellen

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

65669-1-TR bindet in FC Ly-6C und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Ratte / IgG2a
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: k.A.
• Vollständiger Name: lymphocyte antigen 6 complex, locus C1
• GenBank-Zugangsnummer: BC010764
• Gene symbol: Ly-6C
• Gene ID (NCBI): 17067
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2-8°C. Stable for one year after shipment.

Hintergrundinformationen

Ly-6C (lymphocyte antigen 6 complex, locus C) is a 14-17 kDa GPI-linked cell surface antigen that belongs to the Ly-6 family of glycosyl phosphatidylinositol-anchored surface glycoproteins (PMID: 9192663). Ly-6C is expressed on mouse monocytes/macrophages, endothelial cells, granulocytes, thymocytes, and some T cell subsets (PMID: 2849552). The two alleles of Ly-6C, Ly-6C.1 and Ly-6C.2, result in differential expression of this protein on subpopulations of CD4+ T cells (PMID: 15057973; 9126982). Ly-6C has been reported as a marker of memory CD8+ T cells (PMID: 7543536).