Ly-6C Rekombinanter Antikörper

Ly-6C Rekombinant Antikörper für FC

Wirt / Isotyp

Ratte / IgG2a

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

HK1.4

Kat-Nr. : CL488-65669

Synonyme

Ly6c, HK1.4, Ly 6C, Ly6c1

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  FC experiment of mouse bone marrow cells using CL488-65669

  1x10^6 mouse bone marrow cells were surface stained with APC Anti-Mouse CD11b, and 0.25 ug CoraLite® Plus 488 Anti-Mouse Ly-6C (HK1.4) Rat IgG2a RecAb (CL488-65669, Clone: HK1.4) or CoraLite® Plus 488 Rat IgG2a Isotype Control (2A3) (CL488-65209, Clone: 2A3). Cells were not fixed.

• 

  FC experiment of mouse bone marrow cells using CL488-65669

  1x10^6 mouse bone marrow cells were surface stained with 0.25 ug CoraLite® Plus 488 Anti-Mouse Ly-6C (HK1.4) Rat IgG2a RecAb (CL488-65669, Clone: HK1.4) (red) or CoraLite® Plus 488 Rat IgG2a Isotype Control (2A3) (CL488-65209, Clone: 2A3) (blue). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Maus-Knochenmarkzellen

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL488-65669 bindet in FC Ly-6C und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Ratte / IgG2a
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: k.A.
• Vollständiger Name: lymphocyte antigen 6 complex, locus C1
• GenBank-Zugangsnummer: BC010764
• Gene symbol: Ly-6C
• Gene ID (NCBI): 17067
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

Ly-6C (lymphocyte antigen 6 complex, locus C) is a 14-17 kDa GPI-linked cell surface antigen that belongs to the Ly-6 family of glycosyl phosphatidylinositol-anchored surface glycoproteins (PMID: 9192663). Ly-6C is expressed on mouse monocytes/macrophages, endothelial cells, granulocytes, thymocytes, and some T cell subsets (PMID: 2849552). The two alleles of Ly-6C, Ly-6C.1 and Ly-6C.2, result in differential expression of this protein on subpopulations of CD4+ T cells (PMID: 15057973; 9126982). Ly-6C has been reported as a marker of memory CD8+ T cells (PMID: 7543536).