Ly-6G/Ly-6C (Gr-1) Monoklonaler Antikörper

Ly-6G/Ly-6C (Gr-1) Monoklonal Antikörper für FC

Wirt / Isotyp

Ratte / IgG2b, kappa

Getestete Reaktivität

Maus

Anwendung

IF, FC

Konjugation

FITC Plus Fluorescent Dye

Publikationen(4)

CloneNo.

RB6-8C5

Kat-Nr. : FITC-65140

Synonyme

Ly6g, RB6-8C5, Ly-6G, Ly 6G

---

• 

  FC experiment of mouse bone marrow cells using FITC-65140

  1X10^6 mouse bone marrow cells were surface stained with 0.5 ug FITC Plus Anti-Mouse Ly-6G/Ly-6C (Gr-1) (FITC-65140, Clone: RB6-8C5) or 0.5 ug isotype control. Cells were not fixed.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Maus-Knochenmarkzellen

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 1 publications below
• FC: See 3 publications below

Produktinformation

FITC-65140 bindet in IF, FC Ly-6G/Ly-6C (Gr-1) und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Ratte / IgG2b, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Gegen Granulozyten murinen Ursprungs gezüchtet
• Vollständiger Name: lymphocyte antigen 6 complex, locus G
• GenBank-Zugangsnummer: X70920
• Gene symbol: Ly-6G
• Gene ID (NCBI): 546644
• Konjugation: FITC Plus Fluorescent Dye
• Excitation/Emission maxima wavelengths: 495 nm / 524 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

Ly-6G (lymphocyte antigen 6 complex, locus G), also known as Gr-1, is a 21-25 kDa, glycosylphosphatidylinositol-anchored protein expressed on myeloid lineage cells in mouse bone marrow (PMID: 8360469). The expression of Ly-6G increases on neutrophils as they differentiate from immature cells in the bone marrow to mature cells in the blood and spleen (PMID: 8890901). Antibodies targeting Ly6G (RB6-8C5 or 1A8) are commonly used in studies aimed at identifying the role of neutrophils (PMID: 23543767). The RB6-8C5 mAb has been reported to cross-react with Ly-6C on cells expressing Ly-6C (PMID: 8360469).

Publikationen

Species	Application	Title
mouse	FC	Adv Sci (Weinh); Cancer-Associated Fibroblast-Induced Remodeling of Tumor Microenvironment in Recurrent Bladder Cancer; Authors - Ting Liang
mouse	FC	Front Immunol; Endogenous α7 nAChR Agonist SLURP1 Facilitates Escherichia coli K1 Crossing the Blood-Brain Barrier.; Authors - Xiaolong He
mouse	FC	J Nanobiotechnology; The combination of modified acupuncture needle and melittin hydrogel as a novel therapeutic approach for rheumatoid arthritis treatment; Authors - Lisha Liu
mouse	IF	Pharmaceutics; Neutrophil-Camouflaged Stealth Liposomes for Photothermal-Induced Tumor Immunotherapy Through Intratumoral Bacterial Activation; Authors - Xinxin Chen