Ly-6G Rekombinanter Antikörper

Ly-6G Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

242141B11

Kat-Nr. : 98284-1-RR

Synonyme

Ly6g, 242141B11, Ly 6G, Ly-6G.1

---

• 

  FC experiment of mouse bone marrow cells using 98284-1-RR

  1x10^6 mouse bone marrow cells were surface stained with 0.25 ug Anti-Mouse Ly-6G Rabbit RecAb (98284-1-RR, Clone: 242141B11) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Mouse CD11b (M1/70) (CL647-65055, Clone: M1/70). Cells were not fixed.

• 

  FC experiment of mouse bone marrow cells using 98284-1-RR

  1x10^6 mouse bone marrow cells were surface stained with 0.25 ug Anti-Mouse Ly-6G Rabbit RecAb (98284-1-RR, Clone:242141B11) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

• 

  Affinity and Kinetic Characterization of 98284-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98284-1-RR against Mouse Ly-6G were performed. The affinity constant is 0.381 nM.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Maus-Knochenmarkzellen

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

98284-1-RR bindet in FC Ly-6G und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Ly-6G fusion protein Eg2609
• Vollständiger Name: lymphocyte antigen 6 complex, locus G
• Berechnetes Molekulargewicht: 14 kDa
• GenBank-Zugangsnummer: NM_001310438.1
• Gene symbol: Ly-6G
• Gene ID (NCBI): 546644
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2 - 8°C. Stable for one year after shipment.

Hintergrundinformationen

Ly-6G (lymphocyte antigen 6 complex, locus G), also known as Gr-1, is a 21-25 kDa, glycosylphosphatidylinositol-anchored protein expressed on myeloid lineage cells in mouse bone marrow (PMID: 8360469). The expression of Ly-6G increases on neutrophils as they differentiate from immature cells in the bone marrow to mature cells in the blood and spleen (PMID: 8890901).