Lysozyme Rekombinanter Antikörper

Lysozyme Rekombinant Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

241054E1

Kat-Nr. : 84140-1-PBS

Synonyme

LYZ, 1,4-beta-N-acetylmuramidase C, 241054E1, EC:3.2.1.17, Lysozyme C

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  WB analysis of human saliva using 84140-1-RR (same clone as 84140-1-PBS)

  human saliva were subjected to SDS PAGE followed by western blot with 84140-1-RR (Lysozyme antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84140-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 84140-1-RR (same clone as 84140-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84140-1-RR (Lysozyme antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84140-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 84140-1-RR (same clone as 84140-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84140-1-RR (Lysozyme antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84140-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 84140-1-PBS

  Biolayer interferometry (BLl) kinetic assay of 84140-1-PBS against Human Lysozyme was performed. The affinity constant is 116 nM.

• 

  Affinity and Kinetic Characterization of 84140-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84140-1-RR against Human Lysozyme were performed. The affinity constant is 133 nM.

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Geprüfte Anwendungen

Produktinformation

84140-1-PBS bindet in WB, IHC, ELISA Lysozyme und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Lysozyme fusion protein Ag6917
• Vollständiger Name: lysozyme (renal amyloidosis)
• Berechnetes Molekulargewicht: 17 kDa
• Beobachtetes Molekulargewicht: 10 kDa
• GenBank-Zugangsnummer: BC004147
• Gene symbol: Lysozyme
• Gene ID (NCBI): 4069
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LYZ(Lysozyme C) is also named as LZM and belongs to the glycosyl hydrolase 22 family. It catalyzes the hydrolysis of the bacterial cell wall beta(1-4) glycosidic linkages between N-acetylmuramic acid and N-acetylglucosamine. In tissues and body fluids this protein is associated with the monocyte-macrophage system and enhance the activity of immunoagents. LYZ is capable of both hydrolysis and transglycosylation; it shows also a slight esterase activity. Defects in LYZ are a cause of amyloidosis type 8 (AMYL8). The full length protein has a signal peptide with 18 amino acid.