M-cadherin Rekombinanter Antikörper

M-cadherin Rekombinant Antikörper für WB, IHC, IF/ICC, IF-P, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241837C10

Kat-Nr. : 84440-5-PBS

Synonyme

CDH15, 241837C10, Cadherin 14, Cadherin 15, Cadherin-14

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  WB analysis of mouse cerebellum using 84440-5-RR (same clone as 84440-5-PBS)

  mouse cerebellum tissue was subjected to SDS PAGE followed by western blot with 84440-5-RR (CDH15 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84440-5-PBS in a different storage buffer formulation.

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  IHC staining of mouse skeletal muscle using 84440-5-RR (same clone as 84440-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 84440-5-RR (M-cadherin antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84440-5-PBS in a different storage buffer formulation.

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  IF Staining of mouse skeletal muscle using 84440-5-RR (same clone as 84440-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skeletal muscle tissue using M-cadherin antibody (84440-5-RR, Clone: 241837C10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84440-5-PBS in a different storage buffer formulation.

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  IF Staining of C2C12 using 84440-5-RR (same clone as 84440-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using CDH15 antibody (84440-5-RR, Clone: 241837C10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84440-5-PBS in a different storage buffer formulation.

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  IF Staining of H9C2 using 84440-5-RR (same clone as 84440-5-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed H9C2 cells using M-cadherin antibody (84440-5-RR, Clone: 241837C10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84440-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84440-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84440-5-RR against Human M-cadherin were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

84440-5-PBS bindet in WB, IHC, IF/ICC, IF-P, Indirect ELISA M-cadherin und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: M-cadherin fusion protein Ag25763
• Vollständiger Name: cadherin 15, type 1, M-cadherin (myotubule)
• Berechnetes Molekulargewicht: 89 kDa
• Beobachtetes Molekulargewicht: 90-130 kDa
• GenBank-Zugangsnummer: BC008951
• Gene symbol: M-cadherin
• Gene ID (NCBI): 1013
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. M-cadherin (muscle cadherin), also known as CDH15 (cadherin-15), is a 124-kDa type I transmembrane glycoprotein that is highly expressed in developing skeletal muscle, satellite cells, and cerebellum (PMID: 9545347; 12052883). It associates with β-catenin and functions as a cell-cell adhesion molecule in a homophilic and specific manner (PMID: 9545347). M-cadherin is part of the myogenic program and may provide a trigger for terminal muscle differentiation (PMID: 1840697).