M-cadherin Rekombinanter Antikörper

M-cadherin Rekombinant Antikörper für IF/ICC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF/ICC

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

241837C10

Kat-Nr. : CL488-84440-5

Synonyme

CDH15, 241837C10, Cadherin 14, Cadherin 15, Cadherin-14

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  IF Staining of H9C2 using CL488-84440-5

  Immunofluorescent analysis of (4% PFA) fixed H9C2 cells using CoraLite® Plus 488 M-cadherin antibody (CL488-84440-5, Clone: 241837C10 ) at dilution of 1:200.

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  IF Staining of C2C12 using CL488-84440-5

  Immunofluorescent analysis of (-20°C Ethanol) fixed C2C12 cells using CoraLite® Plus 488 M-cadherin antibody (CL488-84440-5, Clone: 241837C10 ) at dilution of 1:200.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: H9C2-Zellen, C2C12-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-84440-5 bindet in IF/ICC M-cadherin und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: M-cadherin fusion protein Ag25763
• Vollständiger Name: cadherin 15, type 1, M-cadherin (myotubule)
• Berechnetes Molekulargewicht: 89 kDa
• Beobachtetes Molekulargewicht: 90-130 kDa
• GenBank-Zugangsnummer: BC008951
• Gene symbol: M-cadherin
• Gene ID (NCBI): 1013
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. M-cadherin (muscle cadherin), also known as CDH15 (cadherin-15), is a 124-kDa type I transmembrane glycoprotein that is highly expressed in developing skeletal muscle, satellite cells, and cerebellum (PMID: 9545347; 12052883). It associates with β-catenin and functions as a cell-cell adhesion molecule in a homophilic and specific manner (PMID: 9545347). M-cadherin is part of the myogenic program and may provide a trigger for terminal muscle differentiation (PMID: 1840697).