MAD2L1 Monoklonaler Antikörper

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MAD2L1 Monoklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2F5F12

Kat-Nr. : 66014-1-PBS

Synonyme

2F5F12, HSMAD2, MAD2, MAD2 like protein 1, MAD2-like protein 1

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  WB analysis of HEK-293 using 66014-1-Ig (same clone as 66014-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66014-1-Ig (MAD2L1 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66014-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66014-1-Ig (same clone as 66014-1-PBS)

  WB result of MAD2L1 antibody (66014-1-Ig, 1:2000) with si-control and si-MAD2L1 transfected HEK293 cells. This data was developed using the same antibody clone with 66014-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66014-1-Ig (same clone as 66014-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66014-1-Ig (MAD2L1 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66014-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 66014-1-Ig (same clone as 66014-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66014-1-Ig (MAD2L1 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66014-1-PBS in a different storage buffer formulation.

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  WB analysis of COLO 320 using 66014-1-Ig (same clone as 66014-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 66014-1-Ig (MAD2L1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66014-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 66014-1-Ig (same clone as 66014-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66014-1-Ig (MAD2L1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66014-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 66014-1-Ig (same clone as 66014-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis slide using 66014-1-Ig (MAD2L1 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 66014-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 66014-1-Ig (same clone as 66014-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis slide using 66014-1-Ig (MAD2L1 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 66014-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66014-1-PBS bindet in WB, IHC, Indirect ELISA MAD2L1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: MAD2L1 fusion protein Ag17512
• Vollständiger Name: MAD2 mitotic arrest deficient-like 1 (yeast)
• Berechnetes Molekulargewicht: 24 kDa
• Beobachtetes Molekulargewicht: 24 kDa
• GenBank-Zugangsnummer: BC000356
• Gene symbol: MAD2L1
• Gene ID (NCBI): 4085
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MAD2 mitotic arrest deficient-like 1 (yeast) (MAD2L1, synonyms: MAD2, HSMAD2) is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2L1 is localized to human chromosome band 5q23.3 by in situ hybridization.