MAD2L1BP Polyklonaler Antikörper

MAD2L1BP Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 15344-1-AP

Synonyme

p31(comet), MAD2L1-binding protein, KIAA0110, CMT2

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  WB analysis using 15344-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15344-1-AP (MAD2L1BP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 15344-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15344-1-AP (MAD2L1BP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 15344-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 15344-1-AP (MAD2L1BP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15344-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15344-1-AP (MAD2L1BP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human skin using 15344-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 15344-1-AP (MAD2L1BP antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skin using 15344-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 15344-1-AP (MAD2L1BP antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human colon using 15344-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 15344-1-AP (MAD2L1BP antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon using 15344-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 15344-1-AP (MAD2L1BP antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of A431 using 15344-1-AP

  Immunofluorescent analysis of A431 cells using 15344-1-AP (MAD2L1BP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, A549-Zellen, HeLa-Zellen, HepG2-Zellen, K-562-Zellen
• Erfolgreiche Detektion in IHC: humanes Hautgewebe, humanes Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

15344-1-AP bindet in WB, IHC, CoIP, ELISA MAD2L1BP und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MAD2L1BP fusion protein Ag7550
• Vollständiger Name: MAD2L1 binding protein
• Berechnetes Molekulargewicht: 34 kDa
• Beobachtetes Molekulargewicht: 34 kDa
• GenBank-Zugangsnummer: BC002904
• Gene symbol: MAD2L1BP
• Gene ID (NCBI): 9587
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MAD2L1BP, also named as CMT2 and KIAA0110, belongs to the MAD2L1BP family. It may function to silence the spindle checkpoint and allow mitosis to proceed through anaphase by binding MAD2L1 after it has become dissociated from the MAD2L1-CDC20 complex. MAD2L1BP interacts with the spindle checkpoint and coordinates cell cycle events in late mitosis. MAD2L1BP may play an active role to remove MAD2 from p55CDC and tether MAD2 for silencing the checkpoint.

Publikationen

Species	Application	Title
human	WB,CoIP	Nat Chem; Multi-tiered chemical proteomic maps of tryptoline acrylamide-protein interactions in cancer cells; Authors - Evert Njomen ; KO Validated