MAD2L2 Monoklonaler Antikörper

MAD2L2 Monoklonal Antikörper für Indirect ELISA, IHC, WB

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus, Ratte

Anwendung

Indirect ELISA, IHC, WB

Konjugation

Unkonjugiert

CloneNo.

2A4C2

Kat-Nr. : 67100-1-PBS

Synonyme

Mitotic arrest deficient 2-like protein 2, MAD2-like protein 2, MAD2B, MAD2 like protein 2, hREV7

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  WB analysis using 67100-1-Ig (same clone as 67100-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67100-1-Ig (MAD2L2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67100-1-PBS in a different storage buffer formulation.

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  WB analysis using 67100-1-Ig (same clone as 67100-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67100-1-Ig (MAD2L2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67100-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 67100-1-Ig (same clone as 67100-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 67100-1-Ig (MAD2L2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67100-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 67100-1-Ig (same clone as 67100-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 67100-1-Ig (MAD2L2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67100-1-PBS in a different storage buffer formulation.

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  FC experiment of K-562 using 67100-1-Ig (same clone as 67100-1-PBS)

  1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human MAD2L2 (67100-1-Ig, Clone:2A4C2) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 67100-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67100-1-PBS bindet in Indirect ELISA, IHC, WB MAD2L2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: MAD2L2 fusion protein Ag28233
• Vollständiger Name: MAD2 mitotic arrest deficient-like 2 (yeast)
• Berechnetes Molekulargewicht: 211 aa, 24 kDa
• Beobachtetes Molekulargewicht: 24 kDa
• GenBank-Zugangsnummer: BC015244
• Gene symbol: MAD2L2
• Gene ID (NCBI): 10459
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MAD family, together with BUB and Mps1,Cdc20k, play roles in the mitotic spindle checkpoint. MAD2L2 is one of the MAD family. It can mediate the second polymerase switching in translation DNA synthesis by mediating the interaction between the error-prone DNA polymerase zeta catalytic subunit REV3L and the inserter polymerase REV1. Through regulation of the JNK-mediate phosphorylation and activation of the transcriptional activator ELK1, MAD2L2 involves in cellular response to DNA damage. Also it has role in the progression of cell cycle and peithelial-mesenchymal transdifferentiation.