MAdCAM1 Polyklonaler Antikörper

MAdCAM1 Polyklonal Antikörper für IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 21917-1-AP

Synonyme

hMAdCAM 1, MACAM1, MAdCAM1

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Galerie der Validierungsdaten

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  IHC staining of human small intestine using 21917-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 21917-1-AP (MAdCAM1 antibody) at dilution of 1:400 (under 10x lens).

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  IHC staining of human small intestine using 21917-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 21917-1-AP (MAdCAM1 antibody) at dilution of 1:400 (under 40x lens).

• 

  IHC staining of human small intestine using 21917-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 21917-1-AP (MADCAM1 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human small intestine using 21917-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 21917-1-AP (MADCAM1 Antibody) at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Dünndarmgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

21917-1-AP bindet in WB, IHC, IF, ELISA MAdCAM1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MAdCAM1 fusion protein Ag16184
• Vollständiger Name: mucosal vascular addressin cell adhesion molecule 1
• Berechnetes Molekulargewicht: 406 aa, 43 kDa
• GenBank-Zugangsnummer: BC142629
• Gene symbol: MADCAM1
• Gene ID (NCBI): 8174
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Lymphocyte recirculation through different organs is thought to be regulated by adhesion molecules ("homing receptors") recognizing tissue-specific vascular addressins on endothelium (PMID: 7511642). The mucosal vascular addressin, MAdCAM1, is an immunoglobulin superfamily adhesion receptor for lymphocytes that is expressed by mucosal venules and helps direct lymphocyte traffic into mucosal tissues including the Peyer patches and the intestinal lamina propria (PMID: 7687523). MAdCAM1 can bind both integrin alpha-4/beta-7 and L-selectin, regulating both the passage and retention of leukocytes.

Publikationen

Species	Application	Title
	IF	Immunity; T Cell Recruitment to the Intestinal Stem Cell Compartment Drives Immune-Mediated Intestinal Damage after Allogeneic Transplantation.; Authors - Ya-Yuan Fu
	WB	Oncotarget; Cytological study on the regulation of lymphocyte homing in the chicken spleen during LPS stimulation.; Authors - Qian Zhang
human	WB,IHC	Cell Death Dis; Integrated DNA and RNA sequencing reveals early drivers involved in metastasis of gastric cancer.; Authors - Jieyun Zhang