MAEA Polyklonaler Antikörper

MAEA Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Rezensionen (2)

Kat-Nr. : 28363-1-AP

Synonyme

EMLP, EMP, HLC 10, MAEA, PIG5

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Galerie der Validierungsdaten

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  WB analysis using 28363-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28363-1-AP (MAEA antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 28363-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28363-1-AP (MAEA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 28363-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28363-1-AP (MAEA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 28363-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28363-1-AP (MAEA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 28363-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28363-1-AP (MAEA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, Jurkat-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IHC: See 1 publications below

Produktinformation

28363-1-AP bindet in WB, IHC, ELISA MAEA und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MAEA fusion protein Ag27150
• Vollständiger Name: macrophage erythroblast attacher
• Berechnetes Molekulargewicht: 45 kDa
• Beobachtetes Molekulargewicht: 45 kDa
• GenBank-Zugangsnummer: BC001225
• Gene symbol: MAEA
• Gene ID (NCBI): 10296
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MAEA(macrophage erythroblast attacher) is a core component of the CTLH E3 ubiquitin-protein ligase complex. MAEA is required for normal cell proliferation and plays a role in erythroblast enucleation during erythrocyte maturation and in the development of mature macrophages. N-terminus MAEA and full-length MAEA are mostly expressed in nuclear and can be detected at low level in the cytoplasm of some cells, while C-terminus MAEA can be detected in the cytoplasm and nucleoli within the nucleus. MAEA mediates the attachment of erythroid cell to mature macrophages which can inhibit erythroid cell apoptosis. The expression of MAEA may be associated with the type 2 diabetes mellitus.(PubMed: 28955747, 24143168, 29911972, 29911972, 30674470, 9763581).

Publikationen

Species	Application	Title
human	WB	Cancers (Basel); Oncopeptide MBOP Encoded by LINC01234 Promotes Colorectal Cancer through MAPK Signaling Pathway.; Authors - Chunyuan Tang
human	WB	Oncogene; E3 ligase MAEA-mediated ubiquitination and degradation of PHD3 promotes glioblastoma progression; Authors - Peijun Zhou ; KD Validated
human	WB	Chembiochem; Identifying E3 ligase substrates with quantitative degradation proteomics; Authors - Victoria N Jordan ; KO Validated
human	WB,IHC	J Transl Med; LAD1 promotes malignant progression by diminishing ubiquitin-dependent degradation of vimentin in gastric cancer; Authors - Yingming Jiang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

S (Verified Customer) (12-31-2021)

Quite a good quality antibody. KO validated.

• Applications: Western Blot
• Cell Tissue Type: HEK293T

MAEA Antibody Western Blot validation ( dilution) in HEK293T (Cat no:28363-1-AP)

FH

H (Verified Customer) (07-09-2020)

This is very good antibody. MAEA band is very thick and clear.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T

MAEA Antibody Western Blot validation (1:1000 dilution) in HEK293T (Cat no:28363-1-AP)