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MAEA Rekombinanter Antikörper

MAEA Rekombinant Antikörper für WB, FC (Intra), Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, FC (Intra), Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

240097B2

Kat-Nr. : 83267-1-PBS

Synonyme

240097B2, Cell proliferation-inducing gene 5 protein, E3 ubiquitin-protein transferase MAEA, EC:2.3.2.27, EMLP

Filter:
  • Western Blot (WB) analysis of various lysates using MAEA Recombinant antibody (83267-1-RR)

    WB analysis using 83267-1-RR (same clone as 83267-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83267-1-RR (MAEA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83267-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of U-251 cells using MAEA Recombinant antibody (83267-1-RR)

    FC experiment of U-251 using 83267-1-RR (same clone as 83267-1-PBS)

    1x10^6 U-251 cells were intracellularly stained with 0.25 ug MAEA Recombinant antibody (83267-1-RR, Clone:240097B2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83267-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83267-1-RR

    Affinity and Kinetic Characterization of 83267-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83267-1-RR against Human MAEA were performed. The affinity constant is 0.496 nM.

Geprüfte Anwendungen

Produktinformation

83267-1-PBS bindet in WB, FC (Intra), Cytometric bead array, Indirect ELISA MAEA und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen MAEA fusion protein Ag27150
Vollständiger Name macrophage erythroblast attacher
Berechnetes Molekulargewicht 45 kDa
GenBank-ZugangsnummerBC001225
Gene symbol MAEA
Gene ID (NCBI) 10296
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.