MAEA Rekombinanter Antikörper

MAEA Rekombinant Antikörper für WB, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

240097E6

Kat-Nr. : 83267-4-PBS

Synonyme

240097E6, Cell proliferation-inducing gene 5 protein, E3 ubiquitin-protein transferase MAEA, EC:2.3.2.27, EMLP

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  WB analysis using 83267-4-RR (same clone as 83267-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83267-4-RR (MAEA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83267-4-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP00233-4

  Sandwich ELISA standard curve of MP00233-4, Human MAEA Recombinant Matched Antibody Pair - PBS only. 83267-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag27150. 83267-4-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL

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  Sample test of MP00233-4

  The mean MAEA concentration was determined to be 38.39 ng/mL in HepG2 cell extract based on a 1.0 mg/mL extract load.

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  IF Staining of HeLa using 83267-4-RR (same clone as 83267-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MAEA antibody (83267-4-RR, Clone: 240097E6 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83267-4-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 83267-4-RR (same clone as 83267-4-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug MAEA Recombinant antibody (83267-4-RR, Clone:240097E6) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83267-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83267-4-PBS

  Biolayer interferometry (BLl) kinetic assay of 83267-4-PBS against Human MAEA was performed. The affinity constant is below 1 pM.

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  Affinity and Kinetic Characterization of 83267-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83267-4-RR against Human MAEA were performed. The affinity constant is 0.283 nM.

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Geprüfte Anwendungen

Produktinformation

83267-4-PBS bindet in WB, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test MAEA und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: MAEA fusion protein Ag27150
• Vollständiger Name: macrophage erythroblast attacher
• Berechnetes Molekulargewicht: 45 kDa
• Beobachtetes Molekulargewicht: 45 kDa
• GenBank-Zugangsnummer: BC001225
• Gene symbol: MAEA
• Gene ID (NCBI): 10296
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MAEA(macrophage erythroblast attacher) is a core component of the CTLH E3 ubiquitin-protein ligase complex. MAEA is required for normal cell proliferation and plays a role in erythroblast enucleation during erythrocyte maturation and in the development of mature macrophages. N-terminus MAEA and full-length MAEA are mostly expressed in nuclear and can be detected at low level in the cytoplasm of some cells, while C-terminus MAEA can be detected in the cytoplasm and nucleoli within the nucleus. MAEA mediates the attachment of erythroid cell to mature macrophages which can inhibit erythroid cell apoptosis. The expression of MAEA may be associated with the type 2 diabetes mellitus.(PubMed: 28955747, 24143168, 29911972, 29911972, 30674470, 9763581).