MAF Polyklonaler Antikörper

MAF Polyklonal Antikörper für IF, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IF, FC (Intra), ELISA

Konjugation

Unkonjugiert

Publikationen

12

Rezensionen (2)

Kat-Nr. : 55013-1-AP

Synonyme

c MAF, cMAF, MAF, Proto oncogene c Maf, Transcription factor Maf

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Galerie der Validierungsdaten

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  WB analysis using 55013-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55013-1-AP (MAF antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 55013-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 55013-1-AP (MAF antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 55013-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 55013-1-AP (MAF antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 55013-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 55013-1-AP (MAF antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of A431 using 55013-1-AP

  IP Result of anti-MAF (IP:55013-1-AP, 4ug; Detection:55013-1-AP 1:500) with A431 cells lysate 2000ug.

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  IF Staining of A431 using 55013-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed A431 cells using 55013-1-AP (MAF antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A375-Zellen, A431-Zellen, HepG2-Zellen, K-562-Zellen
• Erfolgreiche IP: A431-Zellen
• Erfolgreiche Detektion in IF: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 10 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Produktinformation

55013-1-AP bindet in WB, IP, IF, FC (Intra), ELISA MAF und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: v-maf musculoaponeurotic fibrosarcoma oncogene homolog (avian)
• Berechnetes Molekulargewicht: 42 kDa
• Beobachtetes Molekulargewicht: 48-50 kDa
• GenBank-Zugangsnummer: NM_005360
• Gene symbol: MAF
• Gene ID (NCBI): 4094
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MAF, also named as c-Maf, belongs to the bZIP family and Maf subfamily. MAF acts as a transcriptional activator or repressor. It is involved in embryonic lens fiber cell development. MAF increases T cell susceptibility to apoptosis by interacting with MYB and decreasing BCL2 expression. Together with PAX6, it transactivates strongly the glucagon gene promoter through the G1 element. MAF activates transcription of the CD13 proximal promoter in endothelial cells. It is involved in the initial chondrocyte terminal differentiation and the disappearance of hypertrophic chondrocytes during endochondral bone development. When overexpressed, MAF represses anti-oxidant reponse element (ARE)-mediated transcription. It is involved either as an oncogene or as a tumor suppressor, depending on the cell context. A chromosomal aberration involving MAF is found in some forms of multiple myeloma (MM). Defects in MAF are the cause of cataract pulverulent juvenile-onset MAF-related (CAPJOM). Defects in MAF are the cause of cataract congenital cerulean type 4 (CCA4). The antibody is specific to MAF. And it could recognise the 50 kDa band that also be detected in the paper (PMID: 25770584 ) .

Publikationen

Species	Application	Title
mouse	WB	Immunity; RAS P21 Protein Activator 3 (RASA3) Specifically Promotes Pathogenic T Helper 17 Cell Generation by Repressing T-Helper-2-Cell-Biased Programs.; Authors - Bing Wu
human	WB	Acta Pharmacol Sin; Mebendazole elicits potent antimyeloma activity by inhibiting the USP5/c-Maf axis.; Authors - Xue-Han Chen
mouse	WB	Int J Mol Sci; Deletion of Cdk5 in Macrophages Ameliorates Anti-Inflammatory Response during Endotoxemia through Induction of C-Maf and Il-10; Authors - Pauline Pfänder
human	WB	Front Immunol; Molecular Mechanisms Driving IL-10- Producing B Cells Functions: STAT3 and c-MAF as Underestimated Central Key Regulators?; Authors - Magalie Michée-Cospolite
mouse	WB	J Immunol; Malat1 Suppresses Immunity to Infection through Promoting Expression of Maf and IL-10 in Th Cells.; Authors - James P Hewitson
human	WB	Cell Commun Signal; Inhibition of the Otub1/c-Maf axis by the herbal acevaltrate induces myeloma cell apoptosis.; Authors - Tong Sun

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Katie (Verified Customer) (04-08-2022)

Works well for western blot. We have published with this antibody

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Primary CD4 T cells

FH

H (Verified Customer) (04-05-2020)

This protein has a very short half life, and present in the nucleus. I could see a clear band after the nuclear-cytosolic fractionation.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T

MAF Antibody Western Blot validation (1:1000 dilution) in HEK293T (Cat no:55013-1-AP)