c-MAF Polyklonaler Antikörper

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• KD/KO Validated

c-MAF Polyklonal Antikörper für WB, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 55013-1-PBS

Synonyme

MAF, c MAF, cMAF, Proto oncogene c Maf, Proto-oncogene c-Maf

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  WB analysis using 55013-1-AP (same clone as 55013-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 55013-1-AP (c-MAF antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55013-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 55013-1-AP (same clone as 55013-1-PBS)

  WB result of c-MAF antibody (55013-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MAF transfected HepG2 cells. This data was developed using the same antibody clone with 55013-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 55013-1-AP (same clone as 55013-1-PBS)

  WB result of c-MAF antibody (55013-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MAF transfected HeLa cells. This data was developed using the same antibody clone with 55013-1-PBS in a different storage buffer formulation.

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  WB analysis of A375 using 55013-1-AP (same clone as 55013-1-PBS)

  A375 cells were subjected to SDS PAGE followed by western blot with 55013-1-AP (c-MAF antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55013-1-PBS in a different storage buffer formulation.

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  IP experiment of A431 using 55013-1-AP (same clone as 55013-1-PBS)

  IP result of anti-c-MAF (IP:55013-1-AP, 4ug; Detection:55013-1-AP 1:500) with A431 cells lysate 2000ug. This data was developed using the same antibody clone with 55013-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 55013-1-AP (same clone as 55013-1-PBS)

  Immunofluorescent analysis of (10% Formaldehyde) fixed A431 cells using 55013-1-AP (c-MAF antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 55013-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

55013-1-PBS bindet in WB, IF/ICC, IP, Indirect ELISA c-MAF und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: v-maf musculoaponeurotic fibrosarcoma oncogene homolog (avian)
• Berechnetes Molekulargewicht: 42 kDa
• Beobachtetes Molekulargewicht: 42-52 kDa
• GenBank-Zugangsnummer: NM_005360
• Gene symbol: MAF
• Gene ID (NCBI): 4094
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MAF, also named as c-Maf, belongs to the bZIP family and Maf subfamily. MAF acts as a transcriptional activator or repressor. It is involved in embryonic lens fiber cell development. MAF increases T cell susceptibility to apoptosis by interacting with MYB and decreasing BCL2 expression. Together with PAX6, it transactivates strongly the glucagon gene promoter through the G1 element. MAF activates transcription of the CD13 proximal promoter in endothelial cells. It is involved in the initial chondrocyte terminal differentiation and the disappearance of hypertrophic chondrocytes during endochondral bone development. When overexpressed, MAF represses anti-oxidant reponse element (ARE)-mediated transcription. It is involved either as an oncogene or as a tumor suppressor, depending on the cell context. A chromosomal aberration involving MAF is found in some forms of multiple myeloma (MM). Defects in MAF are the cause of cataract pulverulent juvenile-onset MAF-related (CAPJOM). Defects in MAF are the cause of cataract congenital cerulean type 4 (CCA4). The antibody is specific to MAF. And it could recognise the 50 kDa band that also be detected in the paper (PMID: 25770584 ) .