• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

MAFK Polyklonaler Antikörper

MAFK Polyklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 33199-1-PBS

Synonyme

Erythroid transcription factor NF-E2 p18 subunit, Transcription factor MafK

Filter:
  • Western Blot (WB) analysis of various lysates using MAFK Polyclonal antibody (33199-1-AP)

    WB analysis using 33199-1-AP (same clone as 33199-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 33199-1-AP (MAFK antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 33199-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using MAFK Polyclonal antibody (33199-1-AP)

    IF Staining of HeLa using 33199-1-AP (same clone as 33199-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MAFK antibody (33199-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 33199-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

33199-1-PBS bindet in WB, IF/ICC, Indirect ELISA MAFK und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen MAFK fusion protein Ag38230
Vollständiger Name v-maf musculoaponeurotic fibrosarcoma oncogene homolog K (avian)
Berechnetes Molekulargewicht18 kDa，156aa
Beobachtetes Molekulargewicht18 kDa
GenBank-ZugangsnummerNM_002360.3
Gene symbol MAFK
Gene ID (NCBI) 7975
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The small Maf proteins (sMafs) are basic region leucine zipper (bZIP)-type transcription factors. MAFF, MAFG, and MAFK are the three vertebrate sMafs (PMID: 27058431). They act as transcriptional activators by dimerizing with other (usually larger) basic-zipper proteins, such as NFE2, NFE2L1/NRF1, NFE2L2/NRF2, and NFE2L3/NRF3, and recruiting them to specific DNA-binding sites (PMID: 8932385; 9150357). In cancer cell lines, MAFK promotes epithelial-to-mesenchymal transition (EMT) by downregulating E-cadherin expression through the targeting of transmembrane glycoprotein nonmetastatic B (GPNMB), a potent inducer of EMT (PMID: 28400538; 36061142).