MAG Polyklonaler Antikörper

MAG Polyklonal Antikörper für IF, IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

12

Rezensionen (1)

Kat-Nr. : 14386-1-AP

Synonyme

GMA, MAG, myelin associated glycoprotein, S MAG, SIGLEC 4A, SIGLEC4A

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Galerie der Validierungsdaten

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  WB analysis using 14386-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14386-1-AP (MAG antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 14386-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 14386-1-AP (MAG Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

• 

  WB analysis of mouse spinal cord using 14386-1-AP

  mouse spinal cord tissue were subjected to SDS PAGE followed by western blot with 14386-1-AP (MAG Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 14386-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14386-1-AP (MAG antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 14386-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14386-1-AP (MAG antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brain using 14386-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 14386-1-AP (MAG antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 14386-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 14386-1-AP (MAG antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Rückenmarkgewebe, Maushirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:3000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 10 publications below
• IF: See 4 publications below

Produktinformation

14386-1-AP bindet in WB, IHC, IF, ELISA MAG und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MAG fusion protein Ag5845
• Vollständiger Name: myelin associated glycoprotein
• Berechnetes Molekulargewicht: 69 kDa
• Beobachtetes Molekulargewicht: 100 kDa
• GenBank-Zugangsnummer: BC053347
• Gene symbol: MAG
• Gene ID (NCBI): 4099
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MAG (myelin associated glycoprotein) is a 100-kDa transmembrane glycoprotein that is a member of the SIGLEC family of proteins. MAG is localized in periaxonal Schwann cell and oligodendroglial membranes of myelin sheaths where it functions in glia-axon interactions (PMID: 17241126). It is a functional ligand for the Nogo-66 receptor (PMID: 12089450). MAG is a cell adhesion molecule for postnatal neural development and is thought to be involved in the process of myelination.

Publikationen

Species	Application	Title
human	WB	Brain; A role of the frontotemporal lobar degeneration risk factor TMEM106B in myelination.; Authors - Tuancheng Feng
mouse	WB	Acta Neuropathol Commun; TMEM106B deficiency impairs cerebellar myelination and synaptic integrity with Purkinje cell loss.; Authors - Tuancheng Feng
mouse	IF, WB	J Cereb Blood Flow Metab; Connexin43 promotes angiogenesis through activating the HIF-1α/VEGF signaling pathway under chronic cerebral hypoperfusion.; Authors - Weiwei Yu
mouse	WB	Mol Neurobiol; Quercetin Alleviates Demyelination Through Regulating Microglial Phenotype Transformation to Mitigate Neuropsychiatric Symptoms in Mice with Vascular Dementia.; Authors - Zihu Tan
mouse	IF	Neurosci Bull; Digoxin Ameliorates Glymphatic Transport and Cognitive Impairment in a Mouse Model of Chronic Cerebral Hypoperfusion; Authors - Jie Cao
rat	WB	CNS Neurosci Ther; Proteomic analysis of the effects of caffeine in a neonatal rat model of hypoxic-ischemic white matter damage.; Authors - Liu Yang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Emma (Verified Customer) (02-22-2022)

Works well on FFPE prostate tissue by IF. (Tris-EDTA antigen retrieval).

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:50
• Cell Tissue Type: Prostate

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