MAG Monoklonaler Antikörper

MAG Monoklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3H9G3

Kat-Nr. : 66709-1-PBS

Synonyme

GMA, MAG, myelin associated glycoprotein, S MAG, SIGLEC 4A, SIGLEC4A

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  WB analysis of rat brain using 66709-1-Ig (same clone as 66709-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66709-1-Ig (MAG antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66709-1-PBS in a different storage buffer formulation.

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  WB analysis of rat cerebellum using 66709-1-Ig (same clone as 66709-1-PBS)

  rat cerebellum tissue were subjected to SDS PAGE followed by western blot with 66709-1-Ig (MAG antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66709-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 66709-1-Ig (same clone as 66709-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66709-1-Ig (MAG antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66709-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 66709-1-Ig (same clone as 66709-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66709-1-Ig (MAG antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66709-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66709-1-PBS bindet in WB, IHC, Indirect ELISA MAG und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: MAG fusion protein Ag6065
• Vollständiger Name: myelin associated glycoprotein
• Berechnetes Molekulargewicht: 69 kDa
• Beobachtetes Molekulargewicht: 100 kDa
• GenBank-Zugangsnummer: BC053347
• Gene symbol: MAG
• Gene ID (NCBI): 4099
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MAG (myelin associated glycoprotein) is a 100-kDa transmembrane glycoprotein that is a member of the SIGLEC family of proteins. MAG is localized in periaxonal Schwann cell and oligodendroglial membranes of myelin sheaths where it functions in glia-axon interactions (PMID: 17241126). It is a functional ligand for the Nogo-66 receptor (PMID: 12089450). MAG is a cell adhesion molecule for postnatal neural development and is thought to be involved in the process of myelination.