MAG Rekombinanter Antikörper

MAG Rekombinant Antikörper für WB, IHC, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

250555A7

Kat-Nr. : 86097-1-PBS

Synonyme

GMA, myelin associated glycoprotein, S MAG, SIGLEC 4A, SIGLEC4A

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  WB analysis of mouse brain using 86097-1-RR (same clone as 86097-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 86097-1-RR (MAG antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86097-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP02467-1

  Sandwich ELISA standard curve of MP02467-1, Human MAG Recombinant Matched Antibody Pair - PBS only. 86097-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg2975. 86097-1-PBS was HRP conjugated as the detection antibody. Range: 46.9-3000 pg/mL.

• 

  Sample test of MP02467-1

  The mean MAG concentration was determined to be 1.2 ng/mL in SH-SY5Y cell extract based on a 3.2 mg/mL extract load.

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  IHC staining of mouse brain using 86097-1-RR (same clone as 86097-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 86097-1-RR (MAG antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86097-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 86097-1-RR (same clone as 86097-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 86097-1-RR (MAG antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86097-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

86097-1-PBS bindet in WB, IHC, Sandwich ELISA, Indirect ELISA, Sample test MAG und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: MAG fusion protein Eg2975
• Vollständiger Name: myelin associated glycoprotein
• Berechnetes Molekulargewicht: 69 kDa
• Beobachtetes Molekulargewicht: 100 kDa
• GenBank-Zugangsnummer: NM_002361.3
• Gene symbol: MAG
• Gene ID (NCBI): 4099
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MAG (myelin associated glycoprotein) is a 100-kDa transmembrane glycoprotein that is a member of the SIGLEC family of proteins. MAG is localized in periaxonal Schwann cell and oligodendroglial membranes of myelin sheaths where it functions in glia-axon interactions (PMID: 17241126). It is a functional ligand for the Nogo-66 receptor (PMID: 12089450). MAG is a cell adhesion molecule for postnatal neural development and is thought to be involved in the process of myelination.