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MAGEA1 Polyklonaler Antikörper

MAGEA1 Polyklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 55105-1-PBS

Synonyme

Filter:
  • Western Blot (WB) analysis of A375 cells using MAGEA1 Polyclonal antibody (55105-1-AP)

    WB analysis of A375 using 55105-1-AP (same clone as 55105-1-PBS)

    A375 cells were subjected to SDS PAGE followed by western blot with 55105-1-AP (MAGEA1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55105-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using MAGEA1 Polyclonal antibody (55105-1-AP)

    WB analysis of MCF-7 using 55105-1-AP (same clone as 55105-1-PBS)

    MCF7 cells were subjected to SDS PAGE followed by western blot with 55105-1-AP (MAGEA1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55105-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A375 cells using MAGEA1 Polyclonal antibody (55105-1-AP)

    IF Staining of A375 using 55105-1-AP (same clone as 55105-1-PBS)

    Immunofluorescent analysis of A375 cells using 55105-1-AP (MAGEA1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 55105-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

55105-1-PBS bindet in WB, IF/ICC, Indirect ELISA MAGEA1 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Peptid
Vollständiger Name melanoma antigen family A, 1 (directs expression of antigen MZ2-E)
Berechnetes Molekulargewicht 34 kDa
Beobachtetes Molekulargewicht 46 kDa
GenBank-ZugangsnummerNM_004988
Gene symbol MAGEA1
Gene ID (NCBI) 4100
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MAGEA1, also named as CT1.1, MZ2-E, MAGE1 and MAGE1A, is the first gene reported to encode a CTL-defined human tumor antigen. It may play a role in embryonal development and tumor transformation or aspects of tumor progression. Antigen recognized on a melanoma by autologous cytolytic T-lymphocytes. MAGEA1 provided direct evidence for the power of the cDNA expression library as an effective strategy to identify genes encoding tumor antigens recognized by HLA-restricted T cells.(PMID:16951217 ) The antibody is specific to MAGEA1.