MAGEA3 Rekombinanter Antikörper

MAGEA3 Rekombinant Antikörper für WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

240435G9

Kat-Nr. : 83480-5-PBS

Synonyme

240435G9, Antigen MZ2 D, Antigen MZ2-D, Cancer/testis antigen 1.3, CT1.3

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  WB analysis using 83480-5-RR (same clone as 83480-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83480-5-RR (MAGEA3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83480-5-PBS in a different storage buffer formulation.

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  WB analysis using 83480-5-RR (same clone as 83480-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83480-5-RR (MAGEA3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83480-5-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 83480-5-RR (same clone as 83480-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using MAGEA3 antibody (83480-5-RR, Clone: 240435G9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83480-5-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 83480-5-RR (same clone as 83480-5-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug MAGEA3 Recombinant antibody (83480-5-RR, Clone:240435G9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83480-5-PBS in a different storage buffer formulation.

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  FC experiment of Jurkat using 83480-5-RR (same clone as 83480-5-PBS)

  1x10^6 Jurkat cells were intracellularly stained with 0.25 ug MAGEA3 Recombinant antibody (83480-5-RR, Clone:240435G9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83480-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83480-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83480-5-RR against Human MAGEA3 were performed. The affinity constant is 73.7 pM.

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Geprüfte Anwendungen

Produktinformation

83480-5-PBS bindet in WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA MAGEA3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: MAGEA3 fusion protein Ag0265
• Vollständiger Name: melanoma antigen family A, 3
• Berechnetes Molekulargewicht: 314 aa, 35 kDa
• Beobachtetes Molekulargewicht: 45 kDa
• GenBank-Zugangsnummer: BC000340
• Gene symbol: MAGEA3
• Gene ID (NCBI): 4102
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MAGEA3 is a member of the MAGEA family, which belongs to chromosome X-clustered cancer/testis antigens. MAGEA3 is expressed in various human cancers including melanoma, brain, breast, lung, thyroid, colorectal, gastric, pancreatic and ovarian cancer. The aberrant expression of MAGEA3 is associated with poor clinical prognosis in many tumor types. The antibody is specific to MAGEA3.