MAGEA4 Rekombinanter Antikörper

MAGEA4 Rekombinant Antikörper für WB, FC (Intra), Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241558G7

Kat-Nr. : 84248-4-PBS

Synonyme

241558G7, Cancer/testis antigen 1.4, CT1.4, MAGE 4 antigen, MAGE 41

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  WB analysis using 84248-4-RR (same clone as 84248-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84248-4-RR (MAGEA4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84248-4-PBS in a different storage buffer formulation.

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  WB analysis of human testis using 84248-4-RR (same clone as 84248-4-PBS)

  human testis tissue was subjected to SDS PAGE followed by western blot with 84248-4-RR (MAGEA4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84248-4-PBS in a different storage buffer formulation.

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  FC experiment of A431 using 84248-4-RR (same clone as 84248-4-PBS)

  1x10^6 A431 cells were intracellularly stained with 0.25 ug MAGEA4 Recombinant antibody (84248-4-RR, Clone:241558G7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 84248-4-PBS in a different storage buffer formulation.

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  FC experiment of U2OS using 84248-4-RR (same clone as 84248-4-PBS)

  1x10^6 U2OS cells were intracellularly stained with 0.25 ug MAGEA4 Recombinant antibody (84248-4-RR, Clone:241558G7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 84248-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84248-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84248-4-RR against Human MAGEA4 were performed. The affinity constant is 2.03 nM.

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Geprüfte Anwendungen

Produktinformation

84248-4-PBS bindet in WB, FC (Intra), Indirect ELISA MAGEA4 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: MAGEA4 fusion protein Ag3193
• Vollständiger Name: melanoma antigen family A, 4
• Berechnetes Molekulargewicht: 317 aa, 35 kDa
• Beobachtetes Molekulargewicht: 45 kDa
• GenBank-Zugangsnummer: BC017723
• Gene symbol: MAGEA4
• Gene ID (NCBI): 4103
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MAGEA4, also named MAGE4 and CT1.4, is one of the germ cell-specific markers (PMID:16540528). SMAGEA4 is one of the subgroup I MAGEAs members which may play important roles in the immune surveillance of certain tumor types and play important roles during embryogenesis.(PMID:15222021) The antibody is specific to MAGEA4.