MAGEA4 Rekombinanter Antikörper

MAGEA4 Rekombinant Antikörper für WB, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

241558B10

Kat-Nr. : 84248-6-RR

Synonyme

241558B10, Cancer/testis antigen 1.4, CT1.4, MAGE 4 antigen, MAGE 41

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  WB analysis using 84248-6-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84248-6-RR (MAGEA4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 84248-6-RR

  human testis tissue was subjected to SDS PAGE followed by western blot with 84248-6-RR (MAGEA4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of A431 using 84248-6-RR

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using MAGEA4 antibody (84248-6-RR, Clone: 241558B10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  FC experiment of A431 using 84248-6-RR

  1x10^6 A431 cells were intracellularly stained with 0.25 ug MAGEA4 Recombinant antibody (84248-6-RR, Clone:241558B10) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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  FC experiment of U-2 OS using 84248-6-RR

  1x10^6 U-2 OS were intracellularly stained with 0.25 ug MAGEA4 Recombinant antibody (84248-6-RR, Clone:241558B10) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, A375-Zellen, humanes Hodengewebe, U2OS-Zellen
• Erfolgreiche Detektion in IF/ICC: A431-Zellen
• Erfolgreiche Detektion in FC (Intra): A431-Zellen, U-2 OS

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:250-1:1000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84248-6-RR bindet in WB, IF/ICC, FC (Intra), ELISA MAGEA4 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: MAGEA4 fusion protein Ag3193
• Vollständiger Name: melanoma antigen family A, 4
• Berechnetes Molekulargewicht: 317 aa, 35 kDa
• Beobachtetes Molekulargewicht: 45 kDa
• GenBank-Zugangsnummer: BC017723
• Gene symbol: MAGEA4
• Gene ID (NCBI): 4103
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MAGEA4 is a member of the MAGEA family, which belongs to chromosome X-clustered cancer/testis antigens. MAGEA4 is expressed in various human cancers but not in normal tissues besides the testis. High expression of MAGE-A4 in tumors was correlated with poor prognosis in many tumor types.