MAGEA6 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

MAGEA6 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 14602-1-AP

Synonyme

Melanoma-associated antigen 6, MAGE-6 antigen, MAGE6, MAGE3B antigen, CT1.6

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  WB analysis using 14602-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14602-1-AP (MAGEA6 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 14602-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14602-1-AP (MAGEA6 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 14602-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14602-1-AP (MAGEA6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 14602-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14602-1-AP (MAGEA6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 14602-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 14602-1-AP (MAGEA6 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 14602-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 14602-1-AP (MAGEA6 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of A375 using 14602-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A375 cells using MAGEA6 antibody (14602-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushodengewebe, A375-Zellen, Rattenhodengewebe
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A375-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 3 publications below

Produktinformation

14602-1-AP bindet in WB, IHC, IF/ICC, ELISA MAGEA6 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MAGEA6 fusion protein Ag6148
• Vollständiger Name: melanoma antigen family A, 6
• Berechnetes Molekulargewicht: 35 kDa
• Beobachtetes Molekulargewicht: 45-50 kDa
• GenBank-Zugangsnummer: BC067731
• Gene symbol: MAGEA6
• Gene ID (NCBI): 4105
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	IHC	Exp Neurol; Helios modulates the maturation of a CA1 neuronal subpopulation required for spatial memory formation.; Authors - Albert Giralt
human	IHC	Anticancer Res; Tissue Expression of Melanoma-associated Antigen A6 and Clinical Characteristics of Gastric Cancer.; Authors - Miyo Endo
human	WB	Exp Ther Med; MAGEA6 positively regulates MSMO1 and promotes the migration and invasion of oesophageal cancer cells.; Authors - Manyu Liu ; KD Validated
human	IHC	Exp Ther Med; Investigation into the expression levels of MAGEA6 in esophageal squamous cell carcinoma and esophageal adenocarcinoma tissues.; Authors - Jun Hao