MAGED2 Polyklonaler Antikörper

MAGED2 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 15252-1-PBS

Synonyme

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  WB analysis using 15252-1-AP (same clone as 15252-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15252-1-AP (MAGED2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15252-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 15252-1-AP (same clone as 15252-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15252-1-AP (MAGED2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15252-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A431 using 15252-1-AP (same clone as 15252-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 15252-1-AP (MAGED2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15252-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 15252-1-AP (same clone as 15252-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 15252-1-AP (MAGED2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15252-1-PBS in a different storage buffer formulation.

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  IP experiment of HepG2 using 15252-1-AP (same clone as 15252-1-PBS)

  IP result of anti-MAGED2 (IP:15252-1-AP, 4ug; Detection:15252-1-AP 1:1000) with HepG2 cells lysate 3200ug. This data was developed using the same antibody clone with 15252-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 15252-1-AP (same clone as 15252-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15252-1-AP (MAGED2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15252-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 15252-1-AP (same clone as 15252-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15252-1-AP (MAGED2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15252-1-PBS in a different storage buffer formulation.

• 

  IF Staining of MCF-7 using 15252-1-AP (same clone as 15252-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using MAGED2 antibody (15252-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), F-actin was stained using CoraLite®594-phalloidin (red) and DNA was stained by DAPI (blue). This data was developed using the same antibody clone with 15252-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

15252-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA MAGED2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MAGED2 fusion protein Ag6828
• Vollständiger Name: melanoma antigen family D, 2
• Berechnetes Molekulargewicht: 65 kDa
• Beobachtetes Molekulargewicht: 65 kDa
• GenBank-Zugangsnummer: BC000304
• Gene symbol: MAGED2
• Gene ID (NCBI): 10916
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.