MAGED4 Polyklonaler Antikörper

MAGED4 Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC,ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 16809-1-AP

Synonyme

KIAA1859, MAGE D4 antigen, MAGE E1, MAGE E1 antigen, MAGE1, MAGED4, MAGED4A, MAGED4B, MAGEE1, melanoma antigen family D, 4, Melanoma associated antigen D4

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Galerie der Validierungsdaten

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  WB analysis of Jurkat using 16809-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16809-1-AP (MAGED4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 16809-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 16809-1-AP (MAGED4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human gliomas using 16809-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 16809-1-AP (MAGED4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 16809-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 16809-1-AP (MAGED4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 16809-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 16809-1-AP (MAGED4 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human heart using 16809-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 16809-1-AP (MAGED4 Antibody) at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, A549-Zellen
• Erfolgreiche Detektion in IHC: humanes Gliomgewebe, humanes Herzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

16809-1-AP bindet in WB, IHC,ELISA MAGED4 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MAGED4 fusion protein Ag10329
• Vollständiger Name: melanoma antigen family D, 4
• Berechnetes Molekulargewicht: 741 aa, 81 kDa
• Beobachtetes Molekulargewicht: 90-100 kDa
• GenBank-Zugangsnummer: BC081566
• Gene symbol: MAGED4
• Gene ID (NCBI): 728239
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MAGED4, originally termed MAGEE1, is a member of MAGE (Melanoma associated antigen) super-family identified by serial analysis of gene expression (SAGE) technique (PMID: 11406556; 24966945). Three isoforms (MAGED4a, b, and c) were produced by alternative splice in the process of gene transcription (PMID: 11406556). This antibody raised against 1-350 aa of human MAGED4 protein detects a band of 90-100 kDa, which is larger than the calculated molecular weight. The slower electrophoretic mobility may be due to abnormal spatial conformation of MAGED4 protein caused by abundance of proline in composition (PMID: 24966945).