MAGOH Polyklonaler Antikörper

MAGOH Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12347-1-PBS

Synonyme

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  WB analysis of K-562 using 12347-1-AP (same clone as 12347-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 12347-1-AP (MAGOH Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12347-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 12347-1-AP (same clone as 12347-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 12347-1-AP (MAGOH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12347-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 12347-1-AP (same clone as 12347-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 12347-1-AP (MAGOH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12347-1-PBS in a different storage buffer formulation.

• 

  WB analysis of K-562 using 12347-1-AP (same clone as 12347-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 12347-1-AP (MAGOH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12347-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HL-60 using 12347-1-AP (same clone as 12347-1-PBS)

  HL-60 cells were subjected to SDS PAGE followed by western blot with 12347-1-AP (MAGOH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12347-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Raji using 12347-1-AP (same clone as 12347-1-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 12347-1-AP (MAGOH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12347-1-PBS in a different storage buffer formulation.

• 

  IP experiment of K-562 using 12347-1-AP (same clone as 12347-1-PBS)

  IP result of anti-MAGOH (IP:12347-1-AP, 3ug; Detection:12347-1-AP 1:500) with K-562 cells lysate 2400ug. This data was developed using the same antibody clone with 12347-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 12347-1-AP (same clone as 12347-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 12347-1-AP (MAGOH antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12347-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 12347-1-AP (same clone as 12347-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 12347-1-AP (MAGOH antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12347-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

12347-1-PBS bindet in WB, IHC, IP, Indirect ELISA MAGOH und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MAGOH fusion protein Ag3004
• Vollständiger Name: mago-nashi homolog, proliferation-associated (Drosophila)
• Berechnetes Molekulargewicht: 146 aa, 17 kDa
• Beobachtetes Molekulargewicht: 17 kDa
• GenBank-Zugangsnummer: BC018211
• Gene symbol: MAGOH
• Gene ID (NCBI): 4116
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MAGOH, belonging to the mago nashi family, is a component of a splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of a few core proteins and several more peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Core components of the EJC functions to mark the position of the exon-exon junction in the mature mRNA and thereby influences downstream processes of gene expression including mRNA splicing, nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). MAGOH regulates the transcriptional activation of STAT3 by interfering complex formation between STAT3 and a core EJC component Y14.