MALT1 Monoklonaler Antikörper

MALT1 Monoklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF/ICC, FC (Intra), CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

CloneNo.

4D3F6

Kat-Nr. : 66225-1-Ig

Synonyme

4D3F6, EC:3.4.22.-, MALT 1, MALT lymphoma-associated translocation, MLT

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  WB analysis of HeLa using 66225-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66225-1-Ig (MALT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 66225-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66225-1-Ig (MALT1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 66225-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66225-1-Ig (MALT1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 66225-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 66225-1-Ig (MALT1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lymphoma using 66225-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66225-1-Ig (MALT1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 66225-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66225-1-Ig (MALT1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 66225-1-Ig

  Immunofluorescent analysis of (10% Formaldehyde ) fixed HeLa cells using 66225-1-Ig(MALT1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  FC experiment of HeLa using 66225-1-Ig

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug MALT1 Monoclonal antibody (66225-1-Ig, Clone:4D3F6) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Jurkat-Zellen, K-562-Zellen
• Erfolgreiche Detektion in IHC: humanes Lymphomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 2 publications below
• CoIP: See 1 publications below

Produktinformation

66225-1-Ig bindet in WB, IHC, IF/ICC, FC (Intra), CoIP, ELISA MALT1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: MALT1 fusion protein Ag20632
• Vollständiger Name: mucosa associated lymphoid tissue lymphoma translocation gene 1
• Berechnetes Molekulargewicht: 824 aa, 92 kDa
• Beobachtetes Molekulargewicht: 92-100 kDa
• GenBank-Zugangsnummer: BC030143
• Gene symbol: MALT1
• Gene ID (NCBI): 10892
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Mucosa-associated lymphoid tissue lymphoma translocation protein 1 belongs to caspase-like protein superfamily.MALT1 plays a potential role in the regulation of apoptosis and induces NFKB activation and required for the normal development of B cells and for the activation of the IKK complex.It acts as a caspase and mediates additional functions related to T cell activation, cleaving both the ubiquitin editing enzyme,TNFAIP3,and the C terminus of BCL10 .It has two isoforms.Researches showed that inhibition of MALT1 blocks A20 and BCL10 cleavage, reduces NFKB activity, and decreases the expression of NFKB targets BCLXL.

Publikationen

Species	Application	Title
mouse,human	IHC,CoIP	Cell Biol Toxicol; The COP9 signalosome stabilized MALT1 promotes Non-Small Cell Lung Cancer progression through activation of NF-κB pathway; Authors - Yinghui Wang
human	IHC	Front Cell Dev Biol; Targeting MALT1 Suppresses the Malignant Progression of Colorectal Cancer via miR-375/miR-365a-3p/NF-κB Axis.; Authors - Rui Qian