MALT1 Monoklonaler Antikörper

MALT1 Monoklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

4D3F6

Kat-Nr. : 66225-1-PBS

Synonyme

4D3F6, EC:3.4.22.-, MALT 1, MALT lymphoma-associated translocation, MLT

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  WB analysis of HeLa using 66225-1-Ig (same clone as 66225-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66225-1-Ig (MALT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66225-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66225-1-Ig (same clone as 66225-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66225-1-Ig (MALT1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66225-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 66225-1-Ig (same clone as 66225-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66225-1-Ig (MALT1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66225-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 66225-1-Ig (same clone as 66225-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 66225-1-Ig (MALT1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66225-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 66225-1-Ig (same clone as 66225-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66225-1-Ig (MALT1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66225-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 66225-1-Ig (same clone as 66225-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66225-1-Ig (MALT1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66225-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66225-1-Ig (same clone as 66225-1-PBS)

  Immunofluorescent analysis of (10% Formaldehyde ) fixed HeLa cells using 66225-1-Ig(MALT1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66225-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 66225-1-Ig (same clone as 66225-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug MALT1 Monoclonal antibody (66225-1-Ig, Clone:4D3F6) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66225-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66225-1-PBS bindet in WB, IHC, IF/ICC, FC (Intra), ELISA MALT1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: MALT1 fusion protein Ag20632
• Vollständiger Name: mucosa associated lymphoid tissue lymphoma translocation gene 1
• Berechnetes Molekulargewicht: 824 aa, 92 kDa
• Beobachtetes Molekulargewicht: 92-100 kDa
• GenBank-Zugangsnummer: BC030143
• Gene symbol: MALT1
• Gene ID (NCBI): 10892
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Mucosa-associated lymphoid tissue lymphoma translocation protein 1 belongs to caspase-like protein superfamily.MALT1 plays a potential role in the regulation of apoptosis and induces NFKB activation and required for the normal development of B cells and for the activation of the IKK complex.It acts as a caspase and mediates additional functions related to T cell activation, cleaving both the ubiquitin editing enzyme,TNFAIP3,and the C terminus of BCL10 .It has two isoforms.Researches showed that inhibition of MALT1 blocks A20 and BCL10 cleavage, reduces NFKB activity, and decreases the expression of NFKB targets BCLXL.