MAML1 Polyklonaler Antikörper

MAML1 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 55493-1-AP

Synonyme

KIAA0200, Mam 1, Mam1, MAML1, mastermind like 1 (Drosophila), Mastermind like protein 1

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  WB analysis using 55493-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55493-1-AP (MAML1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 55493-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 55493-1-AP (MAML1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 55493-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 55493-1-AP (MAML1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Jurkat-Zellen, Mausherzgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• CoIP: See 1 publications below

Produktinformation

55493-1-AP bindet in WB, CoIP, ELISA MAML1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: mastermind-like 1 (Drosophila)
• Berechnetes Molekulargewicht: 108 kDa
• Beobachtetes Molekulargewicht: 108-120 kDa
• GenBank-Zugangsnummer: NM_014757
• Gene symbol: MAML1
• Gene ID (NCBI): 9794
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MAML1, also named as KIAA0200 and Mam-1 , belongs to the mastermind family. It acts as a transcriptional coactivator for NOTCH proteins. MAML1 has been shown to amplify NOTCH-induced transcription of HES1. It enhances phosphorylation and proteolytic turnover of the NOTCH intracellular domain in the nucleus through interaction with CDK8. MAML1 binds to CREBBP/CBP which promotes nucleosome acetylation at NOTCH enhancers and activates transcription. Induces phosphorylation and localization of CREBBP to nuclear foci. It plays a role in hematopoietic development by regulating NOTCH-mediated lymphoid cell fate decisions. This antibody is specific to MAML1. The calculated molecular weight of MAML1 is 108 kDa, but modified MAML1 is about 120 kDa.

Publikationen

Species	Application	Title
human	WB,CoIP	Int J Biol Sci; Interaction between BEND5 and RBPJ suppresses breast cancer growth and metastasis via inhibiting Notch signaling.; Authors - Yanzhu Shi
human	WB	Discov Oncol; CSF2 promotes chemoresistance in colorectal cancer by regulating Notch pathway; Authors - Hairong Zhou