MAML3 Polyklonaler Antikörper

MAML3 Polyklonal Antikörper für IF, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF,ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 23263-1-AP

Synonyme

CAGH3, ERDA3, GDN, KIAA1816, MAM 2, Mam 3, MAM2, MAML3, mastermind like 3 (Drosophila), Mastermind like protein 3, TNRC3

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 23263-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 23263-1-AP (MAML3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 23263-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 23263-1-AP (MAML3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IF Staining of HEK-293 using 23263-1-AP

  Immunofluorescent analysis of HEK-293 cells using 23263-1-AP (MAML3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen
• Erfolgreiche Detektion in IF: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

23263-1-AP bindet in WB, IF,ELISA MAML3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MAML3 fusion protein Ag19788
• Vollständiger Name: mastermind-like 3 (Drosophila)
• Berechnetes Molekulargewicht: 1133 aa, 122 kDa
• Beobachtetes Molekulargewicht: 122 kDa
• GenBank-Zugangsnummer: BC137131
• Gene symbol: MAML3
• Gene ID (NCBI): 55534
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The three MAML genes are widely expressed in adult tissues but exhibit distinct expression patterns in mouse early spinal cord development. All MAML proteins localize to nuclear bodies, share a conserved basic domain in their N termini that binds to the ankyrin repeat domain of Notch, and contain a transcriptional activation domain in their C termini. MAML3 acts as a transcriptional coactivator for NOTCH proteins and has been shown to amplify NOTCH-induced transcription of HES1.