MAN1A1 Polyklonaler Antikörper

MAN1A1 Polyklonal Antikörper für IF, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IF, ELISA

Konjugation

Unkonjugiert

Rezensionen (1)

Kat-Nr. : 23382-1-AP

Synonyme

Alpha 1,2 mannosidase IA, HUMM3, HUMM9, Man(9) alpha mannosidase, MAN1A1, MAN9, Man9 mannosidase

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Galerie der Validierungsdaten

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  WB analysis using 23382-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23382-1-AP (MAN1A1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of MCF-7 using 23382-1-AP

  IP Result of anti-MAN1A1 (IP:23382-1-AP, 4ug; Detection:23382-1-AP 1:300) with MCF-7 cells lysate 2000ug.

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  IF Staining of HepG2 using 23382-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MAN1A1 antibody (23382-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HepG2-Zellen, Mauslebergewebe, MCF-7-Zellen, Rattenlebergewebe
• Erfolgreiche IP: MCF-7-Zellen
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

23382-1-AP bindet in WB, IP, IF, ELISA MAN1A1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MAN1A1 fusion protein Ag20060
• Vollständiger Name: mannosidase, alpha, class 1A, member 1
• Berechnetes Molekulargewicht: 653 aa, 73 kDa
• Beobachtetes Molekulargewicht: 68 kDa
• GenBank-Zugangsnummer: BC065827
• Gene symbol: MAN1A1
• Gene ID (NCBI): 4121
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MAN1A1, also known as HUMM3, HUMM9, MAN9, belongs to the glycosyl hydrolase 47 family. MAN1A1 is a type II transmembrane protein and catalyzes the hydrolysis of three terminal mannose residues from peptide-bound Man(9)-GlcNAc(2) oligosaccharides. MAN1A1 is a tumor-suppressor and low levels of expression of MAN1A1 correlate with poor prognosis in breast cancer patients (PMID: 29381688).

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Boyan (Verified Customer) (01-13-2023)

This antibody gave a fairly good staining at the Golgi area; however, it also stained microtubules, which is supposed to be nonspecific.

• Applications: Immunofluorescence