MAN1A2 Polyklonaler Antikörper

MAN1A2 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 14049-1-AP

Synonyme

Alpha 1,2 mannosidase IB, MAN1A2, MAN1B

---

• 

  WB analysis of human placenta using 14049-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 14049-1-AP (MAN1A2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  IP experiment of human placenta using 14049-1-AP

  IP result of anti-MAN1A2 (IP:14049-1-AP, 4ug; Detection:14049-1-AP 1:200) with human placenta tissue lysate 4000ug.

• 

  IHC staining of human placenta using 14049-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 14049-1-AP (MAN1A2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human placenta using 14049-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 14049-1-AP (MAN1A2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung using 14049-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 14049-1-AP (MAN1A2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung using 14049-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 14049-1-AP (MAN1A2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human ovary using 14049-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 14049-1-AP (MAN1A2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human ovary using 14049-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 14049-1-AP (MAN1A2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 14049-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 14049-1-AP (MAN1A2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 14049-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 14049-1-AP (MAN1A2 antibody) at dilution of 1:50 (under 40x lens).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Plazenta-Gewebe
• Erfolgreiche IP: humanes Plazenta-Gewebe
• Erfolgreiche Detektion in IHC: humanes Plazenta-Gewebe, humanes Herzgewebe, humanes Lungengewebe, humanes Eierstockgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

14049-1-AP bindet in WB, IP, IHC, ELISA MAN1A2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MAN1A2 fusion protein Ag5240
• Vollständiger Name: mannosidase, alpha, class 1A, member 2
• Berechnetes Molekulargewicht: 641 aa, 73 kDa
• Beobachtetes Molekulargewicht: 73 kDa
• GenBank-Zugangsnummer: BC063300
• Gene symbol: MAN1A2
• Gene ID (NCBI): 10905
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The first group or Class I mannosidase is comprised of α1-2 mannosidases from mammals and yeast with encoded polypeptides of 65-75 kDa. Most of them are Ca2+-dependent enzymes located in the ER and/or Golgi which are inhibited by substrate mimics like kifunensine and deoxymannojirimycin. Their specificity is restricted to α(1-2) linkages, and they are mostly inactive towards artificial substrates like arylmannosides(PMID:10207175).

Publikationen

Species	Application	Title
human	WB	World J Gastroenterol; Hepatitis B virus upregulates host expression of α-1,2-mannosidases via the PPARα pathway.; Authors - Song Hu