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MAN1B1 Monoklonaler Antikörper

MAN1B1 Monoklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3C9F10

Kat-Nr. : 68121-1-PBS

Synonyme

ER alpha 1,2 mannosidase, ER mannosidase 1, ERMan1, MAN1B1, MANA ER

Filter:
  • Western Blot (WB) analysis of HeLa cells using MAN1B1 Monoclonal antibody (68121-1-Ig)

    WB analysis of HeLa using 68121-1-Ig (same clone as 68121-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 68121-1-Ig (MAN1B1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68121-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using MAN1B1 Monoclonal antibody (68121-1-Ig)

    WB analysis using 68121-1-Ig (same clone as 68121-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68121-1-Ig (MAN1B1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68121-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using MAN1B1 Monoclonal antibody (68121-1-Ig)

    WB analysis using 68121-1-Ig (same clone as 68121-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68121-1-Ig (MAN1B1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68121-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using MAN1B1 Monoclonal antibody (68121-1-Ig)

    IF Staining of U2OS using 68121-1-Ig (same clone as 68121-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using MAN1B1 antibody (68121-1-Ig, Clone: 3C9F10 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68121-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

68121-1-PBS bindet in WB, IF/ICC, Indirect ELISA MAN1B1 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Maus / IgG2b
Klonalität Monoklonal
Typ Antikörper
Immunogen MAN1B1 fusion protein Ag28302
Vollständiger Name mannosidase, alpha, class 1B, member 1
Berechnetes Molekulargewicht 80 kDa
Beobachtetes Molekulargewicht80 kDa
GenBank-ZugangsnummerBC002953
Gene symbol MAN1B1
Gene ID (NCBI) 11253
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MAN1B1, also known as ERMAN1, MANA-ER, belongs to the glycosyl hydrolase 47 family. MAN1B1 is involved in glycoprotein quality control targeting of misfolded glycoproteins for degradation, specifically converting Man9GlcNAc to Man8GlcNAc isomer B. At high enzyme concentrations, it further trims the carbohydrates to Man5-6GlcNAc2 (PMID: 18003979). Mutations in MAN1B1 cause autosomal-recessive intellectual disability. This gene has been associated with Rafiq syndrome (PMID: 21763484).