MAN1B1 Rekombinanter Antikörper

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MAN1B1 Rekombinant Antikörper für WB, FC (Intra), Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241817E10

Kat-Nr. : 84588-1-PBS

Synonyme

241817E10, EC:3.2.1.113, Endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase, ER alpha 1,2 mannosidase, ER alpha-1,2-mannosidase

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  WB analysis using 84588-1-RR (same clone as 84588-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84588-1-RR (MAN1B1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84588-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 84588-1-RR (same clone as 84588-1-PBS)

  WB result of MAN1B1 antibody (84588-1-RR; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-MAN1B1 transfected HepG2 cells. This data was developed using the same antibody clone with 84588-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 84588-1-RR (same clone as 84588-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84588-1-RR (MAN1B1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84588-1-PBS in a different storage buffer formulation.

• 

  FC experiment of U2OS using 84588-1-RR (same clone as 84588-1-PBS)

  1x10^6 U2OS cells were intracellularly stained with 0.2 ug MAN1B1 Recombinant antibody (84588-1-RR, Clone:241817E10) and Multi-rAb CoraLite ® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005)(red), or 0.2 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84588-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

84588-1-PBS bindet in WB, FC (Intra), Indirect ELISA MAN1B1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: MAN1B1 fusion protein Ag28302
• Vollständiger Name: mannosidase, alpha, class 1B, member 1
• Berechnetes Molekulargewicht: 80 kDa
• Beobachtetes Molekulargewicht: 80 kDa
• GenBank-Zugangsnummer: BC002953
• Gene symbol: MAN1B1
• Gene ID (NCBI): 11253
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MAN1B1, also known as ERMAN1, MANA-ER, belongs to the glycosyl hydrolase 47 family. MAN1B1 is involved in glycoprotein quality control targeting of misfolded glycoproteins for degradation, specifically converting Man9GlcNAc to Man8GlcNAc isomer B. At high enzyme concentrations, it further trims the carbohydrates to Man5-6GlcNAc2 (PMID: 18003979). Mutations in MAN1B1 cause autosomal-recessive intellectual disability. This gene has been associated with Rafiq syndrome (PMID: 21763484).