MANEA Polyklonaler Antikörper

MANEA Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 21296-1-PBS

Synonyme

Glycoprotein endo-alpha-1,2-mannosidase, Endomannosidase, Endo-alpha mannosidase, Endo alpha mannosidase, ENDO

---

• 

  WB analysis of HEK-293 using 21296-1-AP (same clone as 21296-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 21296-1-AP (MANEA Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21296-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse kidney using 21296-1-AP (same clone as 21296-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 21296-1-AP (MANEA Antibody) at dilution of 1:100 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 21296-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 21296-1-AP (same clone as 21296-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21296-1-AP (MANEA Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 21296-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 21296-1-AP (same clone as 21296-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21296-1-AP (MANEA Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 21296-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HEK-293 using 21296-1-AP (same clone as 21296-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using MANEA antibody (21296-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 21296-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

21296-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA MANEA und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MANEA fusion protein Ag15847
• Vollständiger Name: mannosidase, endo-alpha
• Berechnetes Molekulargewicht: 462 aa, 54 kDa
• Beobachtetes Molekulargewicht: 54 kDa
• GenBank-Zugangsnummer: BC146671
• Gene symbol: MANEA
• Gene ID (NCBI): 79694
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.