MAOA Polyklonaler Antikörper

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• KD/KO Validated

MAOA Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (3)

Anwendung

WB, IHC, IF, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(29)

Kat-Nr. : 10539-1-AP

Synonyme

Amine oxidase [flavin-containing] A, EC:1.4.3.21, MAO A, MAO-A, monoamine oxidase A

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  WB analysis of LNCaP using 10539-1-AP

  LNCaP cells were subjected to SDS PAGE followed by western blot with 10539-1-AP (MAOA antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 10539-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10539-1-AP (MAOA antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 10539-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 10539-1-AP (MAOA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of LNCaP using 10539-1-AP

  IP result of anti-MAOA (IP:10539-1-AP, 4ug; Detection:10539-1-AP 1:5000) with LNCaP cells lysate 880 ug.

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  IHC staining of human pancreas cancer using 10539-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10539-1-AP (MAOA antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 10539-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10539-1-AP (MAOA antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 10539-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10539-1-AP (MAOA antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 10539-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10539-1-AP (MAOA antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 10539-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10539-1-AP (MAOA antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: LNCaP-Zellen, humanes Plazenta-Gewebe
• Erfolgreiche IP: LNCaP-Zellen
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:5000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 24 publications below
• IHC: See 9 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

10539-1-AP bindet in WB, IHC, IF, IP, CoIP, ELISA MAOA und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Affe, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MAOA fusion protein Ag0821
• Vollständiger Name: monoamine oxidase A
• Berechnetes Molekulargewicht: 60 kDa
• Beobachtetes Molekulargewicht: 60 kDa
• GenBank-Zugangsnummer: BC008064
• Gene symbol: MAOA
• Gene ID (NCBI): 4128
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MAOA belongs to the flavin monoamine oxidase family. It catalyzes the oxidative deamination of biogenic and xenobiotic amines and has important functions in the metabolism of neuroactive and vasoactive amines in the central nervous system and peripheral tissues. MAOA preferentially oxidizes biogenic amines such as 5-hydroxytryptamine (5-HT), norepinephrine and epinephrine.

Publikationen

Species	Application	Title
mouse	IF	Cell; In situ identification of cellular drug targets in mammalian tissue.; Authors - Zhengyuan Pang
monkey	WB	Sci Adv; ARMC1 partitions between distinct complexes and assembles MIRO with MTFR to control mitochondrial distribution; Authors - Michael J McKenna
human	WB	Sci Adv; p53 loss activates prometastatic secretory vesicle biogenesis in the Golgi.; Authors - Xiaochao Tan
	WB	Sci Adv; Global ubiquitylation analysis of mitochondria in primary neurons identifies endogenous Parkin targets following activation of PINK1.; Authors - Odetta Antico
		J Clin Invest; A pro-tumorigenic secretory pathway activated by p53 deficiency in lung adenocarcinoma.; Authors - Xiaochao Tan
human	WB,IHC	J Hepatol; Monoamine oxidase A suppresses hepatocellular carcinoma metastasis by inhibiting the adrenergic system and its transactivation of EGFR signaling.; Authors - Jun Li

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