MAOB Polyklonaler Antikörper

MAOB Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

16

Kat-Nr. : 12602-1-AP

Synonyme

MAO B, MAOB, Monoamine Oxidase B, Monoamine oxidase type B

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Galerie der Validierungsdaten

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  WB analysis using 12602-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12602-1-AP (MAOB antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis using 12602-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12602-1-AP (MAOB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 12602-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 12602-1-AP (MAOB Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 12602-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 12602-1-AP (MAOB antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 12602-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 12602-1-AP (MAOB Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 12602-1-AP

  IP result of anti-MAOB(IP:12602-1-AP, 4ug; Detection:12602-1-AP 1:4000) with mouse liver tissue lysate 1840 ug.

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  IHC staining of human gliomas using 12602-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 12602-1-AP (MAOB antibody) at dilution of 1:100 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: L02-Zellen, humanes Lebergewebe, Mauslebergewebe, Rattenlebergewebe
• Erfolgreiche IP: Mauslebergewebe
• Erfolgreiche Detektion in IHC: humanes Gliomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 9 publications below
• IHC: See 4 publications below
• IF: See 6 publications below

Produktinformation

12602-1-AP bindet in WB, IP, IHC, IF, ELISA MAOB und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MAOB fusion protein Ag3253
• Vollständiger Name: monoamine oxidase B
• Berechnetes Molekulargewicht: 520 aa, 59 kDa
• Beobachtetes Molekulargewicht: 59 kDa
• GenBank-Zugangsnummer: BC022494
• Gene symbol: MAOB
• Gene ID (NCBI): 4129
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The MAOB gene encodes a 520-amino acid protein with a molecular mass of 58.8 kD and shows 70% amino acid identity to MAOA. MAOA and MAOB are present in the outer mitochondrial membrane in the central nervous system and peripheral tissues.A comparison of highly purified human placental MAOA and human liver MAOB revealed that the A form of the enzyme is larger by 2 kDa and only one potential N-glycosylation site exists in each protein, with each site in a different relevant position (PMID:3387449).

Publikationen

Species	Application	Title
mouse	IF	Neuron; Social deprivation induces astrocytic TRPA1-GABA suppression of hippocampal circuits; Authors - Yi-Ting Cheng
mouse	IF	EMBO Rep; Regional heterogeneity of astrocyte morphogenesis dictated by the formin protein, Daam2, modifies circuit function.; Authors - Juyeon Jo
human	WB	Br J Pharmacol; Andrographolide alleviates parkinsonism in MPTP-PD mice via targeting DRP1-mediated mitochondrial fission.; Authors - Ji Geng
mouse	WB	Aging (Albany NY); A five-m6A regulatory gene signature is a prognostic biomarker in lung adenocarcinoma patients.; Authors - Xiao Wu
mouse	WB	Aging (Albany NY); Maternal high sugar and fat diet benefits offspring brain function via targeting on the gut-brain axis.; Authors - Dongdong Wang
mouse	WB	Transl Psychiatry; A diet high in sugar and fat influences neurotransmitter metabolism and then affects brain function by altering the gut microbiota.; Authors - Yinrui Guo